Full Description
With its 120 protocols provided in the standard SOP format demanded by many industries, this practical reference covers the whole range of different applications for yeast in both the biotechnology industry and in basic research. In addition, it includes comprehensive tables listing sources and suppliers of the important components found in the protocols, as well as introductory sections on the characteristics of different yeasts. The enclosed CDROM further provides additional protocols not available in the book, electronic versions of the most important protocols and template files for preparing one's own SOPs.
Contents
Editorial Introduction to the selected yeast species Identity testing and storage Whole cell and organelle analysis Working with chromosomes, DNA and RNA Working with proteins Enzyme tests Isolation of metabolites Genetic engineering and analysis of recombinant strains Generation of cell substrates for approved processes and their testing Fermentation and process steps Appendix
