Full Description
This book provides an overview of and introduction to the use of bioluminescence for controlling light-sensing molecules. Different from the standard use of bioluminescence in imaging applications in vitro and in vivo, bioluminescent optogenetics harnesses molecularly produced light to activate the wide range of light-controllable modules available, from ion channels to transcription factors. The book covers the principal biological mechanisms of light emission and light sensing and the palette of tools enabled when combining genetically encoded light production with light sensing for use within and across cells. Through examples covering the main applications, Bioluminescent Optogenetics provides a practical guide for using this powerful platform for neuroscience and beyond.
Contents
Introduction to BioLuminescent OptoGenetics.- Bioluminescence Driving Opsins.- Bioluminescence Driving Non-Opsin Photoreceptors.- Bioluminescence for Cell-to-Cell Communication.- Re-Engineering the Connectome with Photons as Synaptic Transmitters (PhAST).- Interluminescence - Control of Synaptic Communication by Biological Light.- Neurotransmitter Sensor Driven Neuromodulation.- Bioluminescence for Integration of Cellular Processes.- Bioluminescent Activity Dependent (BLADe) Platform.- Bioluminescent Kinase Sensors - BlinKS as optogenetic integrators.- Application of BL-OG Tools in Animal Studies.- Application of BL-OG Tools in Translational and Basic Science.- Application of BL-OG Tools in the Mammalian Brain.- Bioluminescence for Education and Outreach.- Outlook.
