Full Description
This volume details protocols on prion disease from multiple disciplines and highlights the contribution each discipline has made to the understanding of prion disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Prions: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Contents
Purification and Fibrillation of Full-length Recombinant PrP.- Method for Folding of Recombinant Prion Protein to Soluble b-sheet Secondary Structure.- Analysis of Prion Protein Conformation using Circular Dichroism Spectroscopy.- Analysis of Prion Protein Structure using Nuclear Magnetic Resonance Spectroscopy.- Immunodetection of PrPSc using Western Immunoblotting Techniques.- Analysis of miRNA Signatures in Neurodegenerative Prion Disease.- Expression of Heterologous PrP and Prion Propagation in RK13 Cells.- Generation of Infectious Prions and Detection with the Prion Infected Cell Assay.- Analysis of Cellular Prion Protein Endoproteolytic Processing.- Cellular Analysis Adult Neural Stem Cells for Investigating Prion Biology.- Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons.- Methods of Protein Misfolding Cyclic Amplification.- RT-QuIC Assays for Prion Disease Detection and Diagnostics.- A Quick Method to Evaluate the Effect of the Amino Acid Sequence inthe Misfolding Proneness of the Prion Protein.- Insights into Mechanisms of Transmission and Pathogenesis from Transgenic Mouse Models of Prion Diseases.- In vivo Near Infrared Imaging of Neurodegeneration.- Strain Typing of Prion Diseases using in vivo Mouse Models.- Preparation and Immunostaining of the Myenteric Plexus of Prion Infected Mice.- Cell Culture Methods for Screening of Prion Therapeutics.- Real-Time Quaking Induced Conversion for Diagnosis of Prion Disease.- Methods for Molecular Diagnosis of Human Prion Disease.- Molecular sub-typing of PrPres in Human Sporadic CJD Brain Tissue.- Intercellular Prion-like Conversion and Transmission of Cu/Zn Superoxide Dismutase (SOD1) in Cell Culture.
