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Full Description
This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells. Chapters detail current and updated methods for muscle stem cell isolation, culture, molecular analysis, cellular analysis, and reintroduction in vivo as well as protocols for studying myogenic stem cells in non-mammalian model systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Muscle Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Contents
Muscle Stem Cells: A Model System for Adult Stem Cell Biology.- Isolation of Muscle Stem Cells From Mouse Skeletal Muscle.- Primary Mouse Myoblast Purification using Magnetic Cell Separation.- Isolation, Culture, and Immunostaining of Skeletal Muscle Myofibers from Wildtype and Nestin-GFP Mice as a Means to Analyze Satellite Cells.- Characterization of Drosophila Muscle Stem Cell-like Adult Muscle Precursors.- Using Transgenic Zebrafish to Study Muscle Stem/Progenitor Cells.- Muscle Interstitial Cells: A Brief Field Guide to Non-satellite Cell Populations in Skeletal Muscle.- Isolation and Characterization of Vessel-Associated Stem/Progenitor Cells from Skeletal Muscle.- Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture.- Single Cell Gene Expression Profiling of Skeletal Muscle-Derived Cells.- Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle.- Transplantation of Skeletal Muscle Stem Cells.- Simultaneous Measurement of Mitochondrial and Glycolytic Activity in Quiescent Muscle Stem Cells.- Monitoring Autophagy in Muscle Stem Cells.- Mimicking Muscle Stem Cell Quiescence in Culture: Methods for Synchronization in Reversible Arrest.- Methods for Observing and Quantifying Muscle Satellite Cell Motility and Invasion In Vitro.- Effects of Macrophage Conditioned-medium on Murine and Human Muscle Cells: Analysis of Proliferation, Differentiation, and Fusion.- Optimization of Satellite Cell Culture through Biomaterials.- Systematic Identification of Genes Regulating Muscle Stem Cell Self-Renewal and Differentiation.- Bioinformatics for Novel Large Intergenic Non-coding RNA (lincRNA) Identification in Skeletal Muscle Cells.
