Full Description
This
volume summarizes current techniques; ranging from protocols for virus growth,
isolation, quantification and generation of recombinant RSV virus to procedures
for the efficient characterization of the host immune response to RSV
infection. These techniques are used in numerous laboratories around the world
and are the building blocks that support the majority of RSV virus research. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls.
Authoritative and cutting-edge, Humana Respiratory Syncytial Virus:
Methods and Protocols aims to ensure successful results
in the further study of this vital field.
Contents
Human Respiratory Syncytial Virus: An Introduction.- RSV Growth and Quantification by Microtitration and qRT-PCR Assays.- Quantification
of RSV Infectious Particles by Plaque Assay and Immunostaining Assay.- Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays.- Secretory Expression and Purification of Respiratory Syncytial Virus G and F
Proteins in Human Cells.- Development of Human Monoclonal
Antibodiesagainst Respiratory Syncytial Virus Using a High Efficiency Human
Hybridoma Technique.- Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune
Protection.- Host Factors Modulating RSV Infection; Use of Small Interfering RNAs to Probe
Functional Importance.- In Vitro Modelling of RSV Infection and Cytopathogenesis in
Well-Differentiated Human Primary Airway Epithelial Cells (WD-PAECs).- Reverse Genetics of Respiratory Syncytial Virus.- Use of Minigenome Systems to Study RSV
Transcription.- Screening for Host Factors Directly
Interacting with RSV Protein: Microfluidics.- A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particles
to Identify Cellular Factors as Drug Targets.- MicroRNA Profiling from RSV Infected Biofluids, Whole Blood, and Tissue Samples.- Mouse
and Cotton Rat Models of Human Respiratory Syncytial Virus.- In Vivo Assessment of Airway Function in the Mouse Model.- Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus.
