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Full Description
Enzyme Reprogramming, Volume 719 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Practical Considerations for Reaction Development with Vanadium-Dependent Haloperoxidases, Characterization and Engineering of thermostable PLP-dependent alpha-oxoamine synthases (AOSs); versatile C-C bond forming biocatalysts, Thiamine diphosphate Enzymes for Photobiocatalytic Enantioselective C(sp3)-H Acylation, Engineering a PLP-dependent Mannichase, Transferase engineering for SAM analog synthesis using combinatorial library design and high-throughput screening, Biaryl Coupling reactions catalyzed by Cytochrome P450s, and much more.
Other chapters cover Engineering of a-ketoglutarate-dependent amino acid hydroxylases, Repurposing thiamine-dependent enzymes for radical biocatalysis, Directed evolution of nonheme Fe Enzymes for azidation, Directed evolution of P450 dearomatases for catalytic asymmetric radical dearomatization, Genetically encoded artificial photoenzymes for enantioselective [2+2] photocycloadditions, Unmasking the Reverse Activity of Old Yellow Enzymes for Selective Carbonyl Desaturation, and more.
Contents
1. Practical Considerations for Reaction Development with Vanadium-Dependent Haloperoxidases
Kyle Biegasiewicz
2. Characterisation and Engineering of thermostable PLP-dependent alpha-oxoamine synthases (AOSs); versatile C-C bond forming biocatalysts
Dominic J. Campopiano
3. Thiamine diphosphate Enzymes for Photobiocatalytic Enantioselective C(sp3)-H Acylation
Rudi Fasan
4. Engineering a PLP-dependent Mannichase
Yang Hai
5. Transferase engineering for SAM analog synthesis using combinatorial library design and high-throughput screening
Stephan Hammer
6. Biaryl Coupling reactions catalyzed by Cytochrome P450s
Carmela Molinaro
7. Engineering of a-ketoglutarate-dependent amino acid hydroxylases
Hans Renata
8. Repurposing thiamine-dependent enzymes for radical biocatalysis
Xiaoqiang Huang
9. Directed evolution of nonheme Fe Enzymes for azidation
Xiongyi Huang
10. Directed evolution of P450 dearomatases for catalytic asymmetric radical dearomatization
Yang Yang
11. Genetically encoded artificial photoenzymes for enantioselective [2+2] photocycloadditions
Fangrui Zhong and Yuzhou Wu
12. Unmasking the Reverse Activity of Old Yellow Enzymes for Selective Carbonyl Desaturation
Yuxuan Ye