単一分子蛍光分光法ハンドブック Handbook of Single Molecule Fluorescence Spectroscopy

Gell, Chris/ Brockwell, David/ Smith, Alastair

Oxford University Press（2006/08発売）

• 製本 Hardcover:ハードカバー版／ページ数 278 p.／サイズ 82 line drawings
• 言語 ENG
• 商品コード 9780198529422
• DDC分類 543.56

基本説明

A practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function. The techniques have wide applications in biology, and underpin some aspects of nanotechnology and quantum information processing.

Full Description

Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics and photophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analysing the resulting data and exploration of the applications in biophysics. It is ideal for graduate research students and others embarking on work in this exciting field.

Contents

1. Introduction ; 2. Single Molecule Fluorescence Techniques ; 3. Single Molecule Fluorescence Instrumentation ; 4. Preparation of samples for single molecule fluorescence spectroscopy ; 5. Fluorescence spectroscopy of freely diffusing single molecules: examples ; 6. Fluorescence spectroscopy of immobilised single molecules: examples ; 7. The outlook for single molecule fluorescence measurements