Description
COMPREHENSIVE REFERENCE EXPLORING MICROSCOPY TECHNIQUES TO SUPPORT THE STUDY OF LIVING SYSTEMS WITH A UNIQUE MULTISCALE FOCUS
Live Biological Imaging Across Scales presents an overview of the technologies and experimental strategies available for live imaging across scales. Each chapter highlights a different technique, working upwards from small to large scale. Readers will find critical evaluation of these techniques and perspectives on future developments in this evolving field, enabling them to effectively identify techniques that may benefit their research activities.
Written by a team of leading experts and part of the Wiley-Royal Microscopical Society series, Live Biological Imaging Across Scales includes information on:
- Single cells, covering single molecule tracking/FRET, actin, microtubules, intermediate filaments, septins, vesicles, phagosomes, and confined environments
- Multiple cells, covering collective migration, endothelial monolayers, flow platelets, and flow, wound healing, monocyte transmigration, cancer spheroids/tumor models, and organoids
- Organisms, covering Dictyostelium, C. elegans, Drosophila, and mouse-tumors
- Imaging of whole cells individually and collectively in both 2D and 3D platforms
Live Biological Imaging Across Scales is an essential reference for microscopists and experimental biologists, particularly those interested and/or working in the expanding field of live imaging in biology, as well as those working in the commercial microscopy industry supply and development.
Table of Contents
List of Contributors xi
Preface xiii
1 Super-Resolution Imaging 1
2 Peering into the Septin Cytoskeleton –New Insights Unlocked by Microscopy Advances 23
3 Imaging Phagocytosis: From Cells to Molecules 43
4 Imaging Wound Healing Across Scales 65
5 Leukocyte and Cancer Adhesion Live-Cell Imaging 81
6 Bridging the Gap Between In Vitro and In Vivo Studies with Live-Cell Imaging of Organoids 97
7 Tiny Organisms, Big Advantages: Exploring Membrane Damage Responses Using Dictyostelium discoideum in High-Throughput Single-Cell Analyses 123
8 Live Imaging in Caenorhabditis elegans Neurons 141
9 It’s Just a Scratch –Lessons from Epidermal Wound Closure in Drosophila 171
10 Intravital Imaging of Tumors 191
Index 211
