Description
This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who wantto learn more about this exciting and still expanding field.
Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Table of Contents
Introduction to Optical Tweezers: Background, System Designs, and Applications.- Quantifying ATP-Independent Nucleosome Chaperone Activity with Single Molecule Methods.- Protein Tethering for Single-Molecule Force Spectroscopy.- Insect Cell-Based Expression of Cytoskeletal Motor Proteins for Single-Molecule Studies.- Probing Mitotic Chromosome Mechanics Using Optical Tweezers.- A Brief Introduction to Single-Molecule Fluorescence Methods.- Single-Molecule Fluorescence Microscopy in Sensory Cilia of Living Caenorhabditis elegans.- Lattice Light-Sheet Motor-PAINT: A Method to Map the Orientations of Microtubules in Complex Three-Dimensional Arrays.- Fluorescence Microscopy of Nanochannel-Confined DNA.- Single-Molecule FRET X.- Single-Molecule Fluorescence Imaging of DNA Replication Stalling at Sites of Nucleoprotein Complexes.- Measuring Transcription Dynamics of Individual Genes Inside Living Cells.- Single-Molecule FRET Resolved Protein Dynamics from Plasmid to Data in Six Steps.- Atomic Force Microscopy: An Introduction.- Atomic Force Microscopy of Viruses: Stability, Disassembly, and Genome Release.- Unfolding and Refolding Proteins using Single-Molecule AFM.- Visualizing Molecular Dynamics by High-Speed Atomic Force Microscopy.- An Introduction to Magnetic Tweezers.- Surface Functionalization, Nucleic Acid Tether Characterization, and Force Calibration for a Magnetic Tweezers Assay.- Correlated Single-Molecule Magnetic Tweezers and Fluorescence Measurements of DNA-Enzyme Interactions.- Detecting DNA Loops Using Tethered Particle Motion.- Single-Cell Measurements using Acoustic Force Spectroscopy (AFS).- DNA Origami-Based Single-Molecule Force Spectroscopy and Applications.
