Description
This detailed second edition presents a wide variety of marine invertebrate model systems, from cephalochordata to holothurians, along with novel experimental protocols for taking advantage of their unique properties. The techniques range from culturing the organisms to modifying their DNA. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Table of Contents
1. Laboratory Culture and Mutagenesis of Amphioxus (Branchiostoma floridae).- Handling and Manipulation of Gametes and Embryos of the Annelidan Worm Pseudopotamilla occelata.- The Starfish Asterina pectinifera: Collection and Maintenance of Adults and Rearing and Metamorphosis of Larvae.- Experimental Tools to Study Regeneration in the Sea Anemone Nematostella vectensis.- Staining and Tracking Methods for Studying Sponge Cell Dynamics.- Microscopy Studies of Placozoans.- Identification of SH2 Domain-Mediated Protein Interactions that Operate at Fertilization in the Sea Star Patiria miniata.- Marine Nemertean Worms for Immunoblotting Studies of Oocyte Aging.- Recovery of Sea Star Egg Cell Surface Proteins Released at Fertilization.- Quantifying Cell Proliferation During Regeneration of Aquatic Worms.- In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis.- Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond.- Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation.- Transcriptomic Analysis in the Sea Anemone Nematostella vectensis.- RNA Interference on Regenerating Holothurian Gut Tissues.- ATAC-Seq for Assaying Chromatin Accessibility Protocol Using Echinoderm Embryos.- Usage of the Sea Urchin Hemicentrotus pulcherrimus Database, HpBase.- Functional Genomic Studies of Trichoplax adhaerens Neuronal Activity.- A Bioinformatics Tutorial for Comparative Development Genomics in Diverse Meiofauna.
