Description
Originally published as the stand-alone Chlamydomonas Sourcebook, then expanded as the second volume in a three-part comprehensive gold-standard reference, The Chlamydomonas Sourcebook: Organellar and Metabolic Processes has been fully revised and updated to include a wealth of new knowledge and resources for the Chlamydomonas community. It details the tremendous progress recently made with respect to imaging the ultrastructure of cells, dissecting acclimation and biosynthetic responses, and elucidating molecular processes underlying the biology of organelles. In particular, this volume includes exciting new developments in the use of imaging technologies for examining supramolecular organization of the chloroplast, defining mechanisms of branched electron transfer pathways in photosynthesis, dissecting the organization of pyrenoids and CO2 concentration mechanisms, presenting the intricacies associated with acclimation to environmental conditions and providing new insights into dark metabolism and the network of fermentative metabolism.This book thus presents the latest advances in both the research and uses of new experimental approaches and technologies, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, aspects of human disease, acclimation to environmental change, and the biogenesis of cellular complexes.- Describes molecular techniques, analysis of the recently sequenced genome, reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism- Provides methods for Chlamydomonas research and best practices for their applications; this includes methods for cell culture, preservation of cultures, preparation of media, lists of inhibitors, and other additives to culture media, classical genetic manipulation, and new approaches for gene transfer and editing technologies- Assists researchers with common laboratory problems such as contamination
Table of Contents
1. The Comparative, Biochemistry, Genetics and Evolution of Starch Metabolism in Chlamydomonas reinhardtii2. Chlamydomonas Glycerolipid Metabolism3. Nitrogen Metabolism in Chlamydomonas4. Phosphorus and Sulfur Uptake, Assimilation, and Deprivation Responses5. Trace metal nutrition and response to deficiency 6. Sensory photoreceptors in Chlamydomonas7. RubisCo and Carbon assimilation8. Photoproduction of reducing power and the Calvin-Benson Cycle9. Metabolic Networks during Dark Anoxia10. Hydrogenases and hydrogen production11. The mitochondrion: from genome to proteome 12. The chloroplast in a changing environment: from genome to proteome 13. Control of organellar gene expression by nucleus-encoded proteins14. Translation and Protein Synthesis in the chloroplast15. Photosynthesis: Light Harvesting16. Photosystem I and II17. Chloroplast ATP synthase and the cytochrome b6f complex18. The multiple routes of photosynthetic electron transfer in Chlamydomonas reinhardtii19. Assembly of Photosynthetic proteins20. Molecular chaperones and proteases21. Tetrapyrrole biosynthesis and signaling (chlorophyll heme and bilins)22. Carotenoids in Chlamydomonas23. Supramolecular organization of chloroplast membranes24. State transitions25. Photoprotection
