単細胞解析エッセンシャル Essentials of Single-Cell Analysis : Concepts, Applications and Future Prospects

著者名：Tseng, Fan-Gang (EDT)/Santra, Tuhin Subhra (EDT)

Springer（2016/01/21発売）

• 言語：ENG
• ISBN：9783662491164
• eISBN：9783662491188

Description

This book provides an overview of single-cellisolation, separation, injection,lysis and dynamics analysis as well as a study of their heterogeneity usingdifferent miniaturized devices. As an important part of single-cell analysis, differenttechniques including electroporation,microinjection, optical trapping, optoporation, rapid electrokinetic patterningand optoelectronictweezers are described in detail. It presents different fluidicsystems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry)and their integration with sensor technology, optical and hydrodynamic stretchers etc.,and demonstrates the applications of single-cell analysis in systems biology,proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics,biomedicine and drug delivery systems. It also discusses the future challengesfor single-cell analysis, including the advantages and limitations.

This book is enjoyable reading material whileat the same time providing essential information to scientists in academia andprofessionals in industry working on different aspects of single-cellanalysis. 

Dr. Fan-Gang Tseng is a Distinguished Professor ofEngineering and System Science atthe National Tsing Hua University, Taiwan.

Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.

Table of Contents

SingleCell Behavioral Assays for Heterogeneity Studies.- Systems biology in single cells.- Electroporation for single cellanalysis.- Microinjection for single cellanalysis.- Optical tools for single cellmanipulation and analysis.- Optoelectrokinetic manipulationfor cell analysis.- Continuous micro/nano-fluidicdevices for single cell analysis.- Single Cell MechanicalProperties: Label-Free Biomarkers for Cell Status Evaluation.- Cytometry of single cells forbiology and biomedicine.- Single cell genomics andepigenomics.- Single cell Metabolomics.- Applications of cell based drugdelivery systems: use of single cell assay.- Applications of Single CellSequencing in Cancer.- Single Cell Characterization ofMicroalgal Lipid Contents with Confocal Raman Microscopy.- Single Differentiated Neurons fromPluripotent Embryonic Stem Cells: Motor Protein Modelling and NeurodegenerativeDisease.