Description
Techniques in the Behavioral and Neural Sciences, Volume 7: Microdialysis in the Neurosciences focuses on the neurochemical methods employed in behavioral and neural sciences. The selection first elaborates on the introduction to intracerebral microdialysis, quantitative microdialysis, and microdialysis compared with other in vivo release models. Discussions focus on computational methods, post-mortem tissue analysis, perfusion methods, and features, development, and future applications of microdialysis. The text then takes a look at the practical aspects of using microdialysis for determination of brain interstitial concentrations and microdialysis and liquid chromatography. The publication examines the procedures for microdialysis with smallbore HPLC, use of microdialysis in pharmacokinetics and pharmacodynamics, and brain dialysis of monoamines. Topics include significance of monoamine concentrations in dialysates; criteria for brain dialysis of monoamines; distribution of drugs to the interstitium of various tissues; methods to measure the extracellular concentration by microdialysis; and application to studies on drug abuse. The manuscript then elaborates on the feasibility of repeated microdialysis for within-subjects design experiments and microdialysis and automated on-line analysis approach to study central cholinergic transmission in vivo. The text is a dependable reference for readers interested in the use of microdialysis in neurosciences.
Table of Contents
PrefaceList of ContributorsSection I Introduction to Microdialysis Chapter 1 Introduction to Intracerebral Microdialysis 1. Development of Microdialysis 2. Features of Microdialysis 3. Comparisons to Push-Pull Perfusions 4. Principles of Microdialysis 5. The Microdialysis Experiment 6. Future Applications of Microdialysis References Chapter 2 Microdialysis Compared with Other in Vivo Release Models 1. Introduction 2. Post-Mortem Tissue Analysis 3. Perfusion Methods 4. In Vivo Voltammetry Acknowledgment ReferencesSection II Issues of Quantification Chapter 3 Quantitative Microdialysis 1. Introduction 2. Quantitative Microdialysis Models 3. Application 4. Computational Methods 5. Overall Concepts and Comment Appendix I Appendix II Nomenclature References Chapter 4 Practical Aspects of Using Microdialysis for Determination of Brain Interstitial Concentrations 1. Introduction 2. Principle of Microdialysis 3. Measurements with Microdialysis 4. Perfusion Fluids 5. Factors Affecting Recovery 6. Tissue Disturbances Caused by Implantation 7. How to Determine Brain Interstitial Concentration with Microdialysis 8. Transformation of Glutamate and Dopamine Dialysate Concentrations Into Brain Interstitial Concentrations 9. Conclusion ReferencesSection III Methodological Considerations Chapter 5 Microdialysis and Liquid Chromatography 1. Introduction 2. Defining the Problem 3. Liquid Chromatography 4. The Role of Column Length and Diameter 5. The Role of Temperature 6. Automated Fraction Collection, Sampling and Derivatization 7. Gradient Elution 8. Detectors 9. Representative Applications 10. Calibration of Microdialysis Probes 11. Conclusion References Chapter 6 Procedures for Microdialysis with Smallbore HPLC 1. Introduction 2. Smallbore HPLC Procedures 3. Microdialysis Procedures 4. Behavioral Procedures 5. Application to Studies on Drug Abuse References Chapter 7 The Use of Microdialysis in Pharmacokinetics and Pharmacodynamics 1. Introduction 2. Methods to Measure the Extracellular Concentration by Microdialysis 3. Relation Between in Vivo Effect and Interstitial Fluid Concentration of Drug 4. Distribution of Drugs to the Interstitum Of Various Tissues 5. Discussion Acknowledgments References Chapter 8 Brain Dialysis of Monoamines 1. Introduction 2. Criteria for Brain Dialysis of Monoamines 3. Significance of Monoamine Concentrations in Dialysates Acknowledgment References Chapter 9 The Feasibility of Repeated Microdialysis for Within-Subjects Design Experiments: Studies on the Mesostriatal Dopamine System 1. Introduction 2. Acute Effects of Amphetamine on Dopamine Neurotransmission 3. Amphetamine Sensitization 4. The Feasibility of Repeated Microdialysis in Dopamine Systems of Rats 5. Discussion 6. Conclusion Acknowledgments References Appendix A Appendix BSection IV The Application of Microdialysis in the Basic Neurosciences Chapter 10 A Microdialysis and Automated On-Line Analysis Approach to Study Central Cholinergic Transmission in Vivo 1. Introduction 2. Analysis of Choline and Acetylcholine Using HPLC 3. Microdialysis and On-Line Analysis 4.
-
- 洋書電子書籍
- White Bird: A Novel…
-
- 和書
- 広島力
