Full Description
Unique coverage of proteomic and glycomic approaches to better distinguish highly dangerous pathogens, as well as using these to explore novel treatment and prevention options. The editors and authors are either part of a specialized European network initiated to develop fast and reliable detection and therapy options, or are associated with the core military research complex of the United States. With its description of the methods, their advantages and limitations, as well as the principle outcomes, this is a must-have resource for all professionals dealing with BSL3 and/or BSL 4 agents.
Contents
Preface Introduction: Application of proteomic technologies for the analysis of microbial infections (Jiri Stulik and Patrick Butaye) BASIC PROTEOMIC METHODS Separation of proteins and peptides (Ludovit Skultety) Basic mass spectrometric approaches (Lenka Hernychova and Martin Hubalek) Quantitative mass spectrometric approaches (Juraj Lenco and Vojtech Tambor) BN-PAGE of microbial protein complexes (Jiri Dresler and Jana Klimentova) Analysis of Francisella tularensis glycoproteins (Lenka Hernychova and Lucie Balonova) IDENTIFICATION OF PROTEINS AS WELL AS GLYCANS FROM MICROORGANISMS AS CANDIDATE MOLECULES FOR USE IN DETECTION/DIAGNOSIS, THERAPY AND PROPHYLAXIS Comparative proteome analysis of strains with differential virulence (Martin Hubalek and Ivona Pavkova) Analysis of Francisella tularensis acetonitrile extracts (Lenka Hernychova, Martin Hubalek and J. Udrzalova) Analysis of culture filtrate proteins of Francisella tularensis (Klara Konecna and Lenka Hernychova) Lipopolysaccharides of Coxiella burnetii: Chemical composition and structure, and their role in diagnosis of Q Fever (Rudolf Toman and Pavol Vadovic) Mimivirus possess anonymous and unique gene products endowed for antigenic properties (Patricia Renesto and Didier Raoult) Detection of differentially modified pathogen proteins by Western Blot after 2-D gel electrophoresis and identification by MALDI-TOF/TOF (Fred Fack, Julia Kessler, Patrick Pirrotte, Jacques Kremer, Dominique Revets, Wim Ammerlaan and Claude P. Muller) Composition and structure of lipids A of the intracellular bacteria Piscirickettsia salmonis and Coxiella burnetii (Pavol Vadovic, Robert Ihnatko and Rudolf Toman) Proteins of Coxiella burnetii and analysis of their function (Robert Ihnatko, Pavol Vadovic and Rudolf Toman) Subtype and toxin variant identification of Botulinum neurotoxin Type A using proteomics techniques (Suzanne R. Kalb, Jakub Baudys, Theresa J. Smith, James L. Pirkle and John R. Barr) Protein microarrays for antigen discovery (Mohan Natesan, Sarah Keasey and Robert G. Ulrich) MALDI-TOF mass spectrometry for rapid identification of highly pathogenic microorganisms (Peter Lasch and Dieter Naumann) ANALYSIS OF HOST PATHOGEN INTERACTIONS Quantitative proteomic profiling of host - pathogen interactions: The interaction of Francisella tularensis LVS with macrophages using J774.2 cell line (Anetta Hartlova, Marek Link, Juraj Lenco and Jiri Stulik) Proteome analysis of bacterial protein expression after ingestion of microbes by macrophages (Martin Brychta and Ivona Pavkova)
