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Full Description
Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.
Contents
1 Plant Cells and Tissues: Structure—Function Relationships.- 2 Methods for the Cytochemical/Histochemical Localization of Plant Cell/Tissue Chemicals.- 3 Methods in Light Microscope Radioautography.- 4 Some Fluorescence Microscopical Methods for Use with Algal, Fungal, and Plant Cells.- 5 Fluorescence Microscopy of Aniline Blue Stained Pistils.- 6 A Short Introduction to Immunocytochemistry and a Protocol for Immunovisualization of Proteins with Alkaline Phosphatase.- 7 The Fixation of Chemical Forms on Nitrocellulose Membranes.- 8 Dark-Field Microscopy and Its Application to Pollen Tube Culture.- 9 Computer-Assisted Microphotometry.- 10 Isolation and Characterization of Endoplasmic Reticulum from Mulberry Cortical Parenchyma Cells.- 11 Methods for the Identification of Isolated Plant Cell Organelles.- 12 Methods for the Detergent Release of Particle-Bound Plant Proteins.- 13 Scanning Electron Microscopy: Preparations for Diatoms.- 14 Methods for the Ultrastructural Analysis of Plant Cells and Tissues.- 15 Methods for Atomic Force and Scanning Tunneling Microscopies.- 16 Visualization of Golgi Apparatus by Zinc Iodide—Osmium Tetroxide (ZIO) Staining.- 17 Methods in Electron Microscope Autoradiography.- 18 Electron Microscopic Immunogold Localization.- 19 Microanalysis.- 20 The Use of Electron Microscopy in Molecular Biology.- 21 Summation.
