Full Description
This volume discusses the latest techniques and methodologies used in the neurotrophin field to study the physiology of the Brain-Derived Neurotrophic Factor (BDNF). The book provides the tools essential to any researcher that intends to explore the peculiar regulation of this neurotrophin from gene expression to its release and the signaling by TrkB receptors, as well as to study the neuronal responses to BDNF or the role of neurotrophin in various neurological diseases. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Cutting edge and comprehensive, Brain-Derived Neurotrophic Factor(BDNF) is a valuable resource for researchers who want to further study the diversity of physiological roles of this important growth factor.
Contents
Brain Derived Neurotrophic Factor and the "Attività plastic dei neuroni", the Neuronal Plasticity as Defined by Ernesto Lugaro (1870-1940).- Usage of Bacterial Artificial Chromosomes for Studying BDNF Gene Regulation in Primary Cultures of Cortical Neurons and Astrocytes.- Detecting Single and Multiple BDNF Transcripts In Situ in Neuronal Cultures and Brain Sections.- Studying BDNF/TrkB Signaling: Transcriptome Analysis from a Limited Number of Purified Adult or Aged Murine Brain Neurons.- Studying BDNF/TrkB Signaling: High-Throughput Microfluidic Gene Expression Analysis from rare of Limited Samples of Adult and Aged Central Neurons.- Detecting BDNF Protein Forms by ELISA, Western Blot, and Immunofluorescence.- Methodology for Detecting and Tracking brain-Derived Neurotrophic Factor Complexes in Neurons using Single Quantum Dots.- Recording Activity-Dependent Release of BDNF from Hippocampal Neurons.- Ultrastructural Localization of BDNF and trkB Receptors.- Analysis of TrkB Receptor Activity using FRET Sensors.- BDNF-Induced Intracellular Signaling.- A Microfluidic Culture Platform for Neurotrophin Signaling Studies.- BDNF-Induced Local Protein Synthesis in Synaptoneurosomes Assessed with Click-iT L-azidohomoalanine.- Proteomic Tools to Study the Effect of BDNF on de novo Protein Synthesis.- BDNF Function in Long-Term Synaptic Plasticity in the Dentate Gyrus in Vivo: Methods for Local Drug Delivery and Biochemical Analysis of Translation.
