Full Description
This volume presents methods for the analysis of genomic variability in vertebrate neurons and broadens our knowledge in the ways we understand the brain and its neurons. The chapters in this book are divided into 5 parts, and cover the following topics: principles and approaches for discovery of somatic mosaicism in the brain, aneuploidy and ploidy variation, DNA copy number variation, LINE-1 retrotransposition, and genetic and genomic mosaicism in aging and disease. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Cutting-edge and authoritative, Genomic Mosaicism in Neurons and Other Cell Types is a valuable resource for learning about the latest techniques for the analysis of genome and genetic mosaicism in vertebrate neurons.
Contents
Principles and Approaches for Discovery and Validation of Somatic Mosaicism in the Human Brain.- FISH-Based Assays for Detecting Genomic (Chromosomal) Mosaicism in Human Brain Cells.- Flow Cytometric and Sorting Analyses for Nuclear DNA Content, Nucleotide Sequencing and Interphase FISH.- Flow Cytometric Quantification, Isolation, and Subsequent Epigenetic Analysis of Tetraploid Neurons.- A Cytomic Approach towards Genomic Individuality of Neurons.- Single Cell CNV Detection in Human Neuronal Nuclei.- Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) for the Analysis of DNA Copy Number Variation.- Competitive PCR for Copy Number Assessment by Restricting dNTPs.- Using Cloning to Amplify Neuronal Genomes for Whole Genome Sequencing and Comprehensive Mutation Detection and Validation.- Analysis of LINE-1 Retrotransposition in Neural Progenitor Cells and Neurons.- Estimation of LINE-1 Copy Number in the Brain Tissue and Isolated Neuronal Nuclei.- Analysis of Somatic LINE-1 Insertions in Neurons.- Single-Cell Whole Genome Amplification and Sequencing to Study Neuronal Mosaicism and Diversity.- FISH Analysis of Aging-Associated Aneuploidy in Neurons and Non-Neuronal Brain Cells.- Genomic Analysis and In Vivo Functional Validation of Brain Somatic Mutations Leading to Focal Cortical Malformations.- Using Fluorescence In Situ Hybridization (FISH) Analysis to Measure Chromosome Instability and Mosaic Aneuploidy in Neurodegenerative Diseases.- Identification of Low Allele Frequency Mosaic Mutations in Alzheimer Disease.
