- ホーム
- > 洋書
- > 英文書
- > Science / Mathematics
Full Description
This volume is the result of an explosion of
molecular-based research on Cilia, which began with the discovery of the
universality of intraflagellar transport (IFT) and ciliary genomics/proteomics.
The chapters in this book cover topics such as: high resolution imaging and
functional characterization of sensory and primary cilia in mammalian cells and
zebrafish, methods to study ciliary-mediated chemoresponse in Paramecium,
and methods to study centrosomes and cilia in C. elegans and Drosophila.
Written in the highly successful Methods in Molecular Biology series format,
chapters include introduction to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Cilia:
Methods and Protocols is broad and covers motile, sensory, and primary
cells. It is a valuable resource to anyone interested in entering the field of
ciliary biology using model organisms, including flagellate algae, ciliates,
planaria, nematodes, insects, zebrafish, and mammalian cells.
Contents
Methods for Studying Ciliary Import Mechanisms.- Targeting of ASH Domain-Containing Proteins to
the Centrosome.- Morphological and Functional Characterization
of the Ciliary Pocket by Electron and Fluorescence Microscopy.- Methods to Study Interactions between Ciliogenesis
and Autophagy.- Recombinant Reconstitution and Purification of
the IFT B Core Complex from Chlamydomonas
reinhardtii.- Methods for Studying Movement of Molecules
within Cilia.- A FRAP-Based Method for Monitoring Molecular Transport
in Ciliary Photoreceptor Cells In Vivo.- Kymographic
Analysis of Transport in an Individual Neuronal Sensory Cilium in Caenorhabditis elegans.- Visualization and Manipulation of Cilia and Intraciliary
Calcium in the Zebrafish Left-Right Organizer.- Methods for Studying Ciliary-Mediated Chemoresponse
in Paramecium.- STED
and STORM Superresolution Imaging of Primary Cilia.- CLEM Methods forStudying Primary Cilia.- Methods for Visualization of Neuronal Cilia.- Methods to Study Centrosomes and Cilia in Drosophila.- Analysis of Axonemal Assembly during Ciliary Regeneration
in Chlamydomonas.- Planaria as a
Model System for the Analysis of Ciliary Assembly and Motility.
