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Full Description
This volume covers protocols related to both pluripotent and somatic stem cells, including the ethical procurement of tissues and cells for the provision of "seed stock," standardized methods for deriving hESCs and iPSCs, isolating mesenchymal stem cells, cell culture and cryopreservation, in addition to quality assurance and information management. Stem Cell Banking: Concepts and Protocols aims to contribute to the development of this field by providing information that is essential to establishing a bona fide stem cell bank. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Stem Cell Banking: Concepts and Protocols is a valuable resource for stem cell scientists and novices to the field, and will help strengthen and maximize their use of existing and future stem cell resources.
Contents
Stem Cell Banking: A Global View.- Quality Assurance in Stem Cell Banking: Emphasis on Embryonic and Induced Pluripotent Stem Cell Banking.- Acquisition and Reception of Primary Tissues, Cells, or Other Biological Specimens.- Information Management.- Cryopreservation: Vitrification and Control Rate Cooling.- Quality Assured Characterization of Stem Cells for Safety in Banking for Clinical Application.- Ethics and Governance of Stem Cell Banks.- Derivation of Human Embryonic Stem Cells.- Derivation of Human Induced Pluripotent Stem Cells in Chemically Defined Medium.- Culture, Adaptation, and Expansion of Pluripotent Stem Cells.- Cryobanking Pluripotent Stem Cells.- Genome Editing in Human Pluripotent Stem Cells.- Isolation, Culture, and Expansion of Mesenchymal Stem Cells.- Cryobanking Mesenchymal Stem Cells.- Culturing and Cryobanking Human Neural Stem Cells.
