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Full Description
This volume details up-to-date information and attempts to take the reader into the exciting realm of TGF- from the basic principles to the practical applications. Chapters provide basic introduction of TGF- signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF- signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway; the physiological implications including those in cell cycle arrest, epithelial-mesenchymal transition, endothelial cells, adipogenesis, Th differentiation, stem cell, bone remodeling, ovary, zebrofish development, and frog animal capping; and the methodologies including metastasis imaging, 3D morphogenesis, membrane receptor quantification, conditional knockout, bone remodeling, kinase and phosphatase assays, BiFC interaction assays, and genome-wide siRNA screen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, TGF-Beta Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Contents
1. Regulation of TGF-b ReceptorsErine Budi, Jian Xu and Rik Derynck2. Determining TGF- Receptor Levels in the Cell MembraneLong Zhang, Fangfang Zhou, Maarten van Dinther, and Peter ten Dijke3. Post-translational Modifications of TGF-b ReceptorsXiaohua Yan and Ye-Guang Chen 4. Production, Isolation,and Structural Analysis of Ligands and Receptors of the TGF-b SuperfamilyTao Huang and Andrew P. Hinck 5. Phosphorylation of Smads by Intracellular KinasesFang Liu and Isao Matsuura6. Analysis of Smad Phosphatase Activity in vitroTao Shen, Lan Qin and Xia Lin7. Three-dimensional Mammary Epithelial Cell Morphogenesis Model for Analysis of TGF-b SignalingJuliet Rashidian and Kunxin Luo8. TGF- Signaling in Stem Cell RegulationWenlin Li, Wanguo Wei, Sheng Ding9. Analysis of Epithelial-Mesenchymal Transition Induced by Transforming Growth Factor Ulrich Valcourt, Jonathon Carthy, Yukari Okita, Lindsay Alcaraz, Mitsuyasu Kato, Sylvie Thuault, Laurent Bartholin and Aristidis Moustakas10. In vitro Th differentiation protocolTakashi Sekiya and Akihiko Yoshimura11. Interrogating TGF-b Function and Regulation in Endothelial CellsJ.A. Maring, L.A. van Meeteren, M.J. Goumans and P. ten Dijke12. Isolation and Manipulation of Adipogenic Cells to Assess TGF-b Superfamily FunctionsMaria Namwanje, Juan .C. Bournat and Chester .W. Brown13. Imaging TGFb Signaling in Mouse Models of Cancer MetastasisYibin Kang14. Generation and Characterization of Smad7 Conditional Knockout MiceYi Pan and Yan Chen15. Monitoring Smad Activity in vivo Using the Xenopus Model SystemMarco Montagner, Graziano Martello and Stefano Piccolo16. Animal Cap Assay for TGF-b SignalingChenbei Chang17. Detection of Smad Signaling in Zebrafish EmbryosXingfeng Liu, Qiang Wang and Anming Meng18. Role of TGF- signaling in Coupling Bone RemodelingJanet L. Crane, Lingling Xian and Xu Cao19. Studying the Functions of TGF- Signaling in the OvaryChao Yu, Jian-Jie Zhou and Heng-Yu Fan20. Quantitative Real-Time PCR Analysis of MicroRNAs and Their Precursors Regulated by TGF-b SignalingHara Kang and Akiko Hata21. TGF- Regulated microRNAs and Their Function in Cancer BiologyPengyuan Yang, Yun Zhang, Geoffrey J. Markowitz, Xing Guo and Xiao-Fan Wang22. Epigenomic Regulation Of Smad1 Signaling During Cellular Senescence Induced by Ras ActivationAtsushi Kaneda, AyaNonaka, Takanori Fujita, Ryota Yamanaka, Mai Fujimoto, Kohei Miyazono and Hiroyuki Aburatani23. The Role of Ubiquitination to Determine Non-Smad Signaling ResponsesShyam Kumar Gudey and Marene Landstroem24. Genome-wide RNAi Screening to Dissect the TGF-b Signal Transduction PathwayXiaochu Chen and LanXu25. Measuring TGF- Ligand Dynamics in Culture MediumZipei Feng and Xuedong Liu
