Full Description
A panel of outstanding investigators surveys and explains the major cutting-edge methods used in thryroid receptor (TR) research and explains their practical experimental details. Described in step-by-step detail to ensure robust experimental results, the techniques presented cover a wide variety of key areas, including TR in development and knockout (mouse and Xenopus), transcriptional regulation by TRs in both cell-free systems and in living cells, and TR mutant analysis of patients. Additional methods provide powerful tools for the isolation of TR-regulated protein complexes, for studying the oncogene v-Erba in blood cell differentiation, and for target gene analysis in the brain. Microarray chip methods are also presented for analyzing the organs of transgenic mice to identify target genes in the liver.
Contents
to Thyroid Hormone Receptors.- Null Mutant Mice for Thyroid Hormone Receptors.- Transgenic Targeting of a Dominant Negative Corepressor to Liver and Analyses by cDNA Microarray.- The Role of Thyroid Hormone Receptors in the Heart.- Analysis of Thyroid Hormone-Dependent Genes in the Brain by In Situ Hybridization.- The v-erbA Oncogene.- Gene Regulation by Thyroid Hormone Receptors.- Molecular Analysis of Human Resistance to Thyroid Hormone Syndrome.- Function of Thyroid Hormone Receptors During Amphibian Development.- Transcriptional Regulation by Thyroid Hormone Receptor in Chromatin.- Gene Activation by Thyroid Hormone Receptor In Vitro and Purification of the TRAP Coactivator Complex.
