- ホーム
- > 洋書
- > 英文書
- > Science / Mathematics
Full Description
This detailed volume presents experimental and computational methods for constructing and interrogating synthetic gene circuits across multiple organizational scales. Beginning with a section on genetic tools, platforms, and experimental frameworks that enable sophisticated circuit construction, the book continues by covering methods for regulating circuit behavior and mitigating variability, as well as frameworks for understanding time-dependent circuit behavior in growing cells and populations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Synthetic Gene Circuits: Methods and Protocols aims to support a synthetic biology practice that is predictive by design, robust by control, and interpretable through dynamics.
Contents
Reimagining the Synthetic Biology DBTL Cycle with Machine Learning.- A Golden Gate Compatible CRISPR-Associated Transposon Tool for Multiplexed Bacterial Genome Editing.- Construction of a Tl-CRISPRi Genetic Circuit in Bacteria for Translation-Level Gene Knockdown.- Design of CRISPRi-Based Synthetic Gene Circuits in Bacteria.- Design of Conditional Guide RNAs for the Logical Regulation of Gene Expression.- Synthetic Biomolecular Condensates: Design Principles and Applications.- Characterizing Optogenetic Tools for Use in Synthetic Gene Circuits.- Characterization of Synthetic Gene Circuits with Absolute Quantification in Continuous Culture.- Cell-Free Genetic Circuits: Extract and Template Preparation.- Programming RNA Trans-Splicing for Versatile Gene Regulation and Complex Cellular Logic Computation.- Intercellular CRISPRi for Distributed Genetic Circuits.- Feedback Control and Sensitivity Analysis in Synthetic Gene Circuits.- Dynamic Robust Control of Microbial Communities Using Cybergenetics.- From Negative Feedback to Integrated Control: Recombinase-Based Strategies for Mitigating Resource Competition.- Harnessing CRISPRi Competition to Develop Multimodule Controllers for Resource-Aware Circuit Design.- Modeling Growth-Mediated Dilution in Synthetic Circuits: Effects of Circuit Topology and Phase Separation.- Quantitative Coarse-Grained Modeling of Microbial Metabolism: Uncovering Growth Laws, Environmental Responses, and Host-Circuit Coupling.- Gene Expression Across Growth Stages (GEAGS): Modeling Circuit Dynamics in Batch Cultures.- Coupled Perturbations of Gene Circuit Dynamics by Resource Competition and Growth Dilution.- Dynamic Constrained Allocation Flux Balance Analysis (dCAFBA).- Constructing an NF-κB Oscillator for Dual-Input Control in Yeast.
