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Full Description
This volume looks at the newest technology and approaches, along with classical methodologies, to thoroughly study Glycoscience and Ganglioside science. The chapters in this book cover topics such as synthesis and degradation of gangliosides including chemical synthesis, substrate preparation, and synthetic and degradation enzyme; ganglioside behaviors and biology including neutral glycolipids, single particle tracking, lipid raft analysis, and metabolic analysis in foods; and disease and tissue development including bone metabolism, melanocyte gangliosides, autoimmune neuropathies, and amyloid fibril formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Gangliosides: Methods and Protocols is a valuable resource for researchers and scientists who want to learn more about this important and developing field.
Contents
Chemical Synthesis of Gangliosides: Synthesis of Uniform Ganglioside.- Chemical Synthesis of Fluorescent Analogs of a- and b-Series Gangliosides.- Identification and Evaluation of CMP-Sialic Acids.- Enzyme Activity of Glycolipid Specific Glycosyltransferase Enzymes GM2/GD2 Synthase β1,4-GalNAc Transferase (B4GALNT1).- Degradation System: Sialidase.- LC-MS-Based Analytical Strategies for Ganglioside Profiling.- Structure Analysis of Gangliosides: Mass Spectrometry Imaging.- NMR of Gangliosides.- Analysis of Ganglioside Molecular Species Focusing on Ceramide Structures Using Thin-Layer Chromatography-Mass Spectrometry.- Chemical Identification of Glycosphingolipid Antigens Recognized by Antibodies.- Elucidation of Ganglioside Microdomains Using Proximity Labeling.- Analysis of Intracellular Trafficking of Cholera Toxin B Subunits Depending on Ganglioside GM1 in Tissue Resident Macrophages.- Electrostatic Interaction Analysis Between GM3 and Insulin Receptor.- Methods for Investigating Siglec-Ganglioside Interactions.- Expression of Mono- and Dimeric Ganglioside-Binding Lectins and Their Cellular Functions.- Visualization of Ganglioside-Bacteria Interaction Using a Frozen Tissue Overlay Assay with GFP-Labeled Bacteria and Ganglioside-Specific Antibodies.- Identification of Gangliosides as Bacterial Attachment Sites Using a TLC-Overlay Assay with Radioisotope-Labeled Bacteria.- Analyzing Molecular Complexes Containing Gangliosides via Co-Immunoprecipitation.- Analysis of Lacto/Neolacto-Series Glycolipids and Gangliosides.- Ganglioside Functions in Extracellular Vesicles as Revealed by Single-Particle Tracking.- Small-Scale Cell Biological Analysis of Lipid Rafts of Blood Platelets and Cerebellar Neurons.- Gangliosides and Membrane Microdomains.- Metabolic Analysis: Tracing Gangliosides and Precursors in Foods.- Flow Cytometry Analysis of Gangliosides Expressed on Extracellular Vesicles.- Methods for Evaluation of the Differentiation to Osteoblasts and Osteoclasts: Roles of Gangliosides in Bone Metabolisms.- Ganglioside Expression in Melanocytes: Modulation by Intrinsic and Extrinsic Factors.- Gangliosides and Autoimmune Neuropathies.- Imaging and Characterization of Ganglioside-Induced Amyloid Fibril Formation.- Preparation of Gangliosides-Reconstituted Liposomes.- Intracerebroventricular Administration of Gangliosides for In Vivo Functional Studies in Mice.- Gangliosides in Neural Stem Cell Fate Determination and Nerve Cell Specification: Preparation and Administration.
