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Full Description
This fully updated book explores cerebrospinal fluid (CSF) molecular profiles in normal and pathological states, with a focus on mass spectrometry and different protein methods currently used in neurobiological disciplines. The book includes CSF sample preparation methods, shotgun and targeted proteomic workflows, as well as methods to characterize specific posttranslational modifications to achieve finer molecular resolution in proteomic studies of CSF. Additional chapters delve into experimental -omics strategies applied to the CSF lipidome and metabolome, as well as bioinformatic pipelines useful for analyzing and integrating the molecular information derived from high-throughput -omics experiments. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Cerebrospinal Fluid (CSF) Proteomics: Methods and Protocols, Second Edition serves as an ideal resource for researchers beginning their journey into CSF molecular profiles, as well as for established researchers seeking valuable insight into the growing utility of proteomics and metabolomics in neuroscience.
Contents
Recent Advances in Human Cerebrospinal Fluid Proteomics.- Using Real-Time Quaking-Induced Conversion (RT-QuIC) in Clinical Practice for Creutzfeldt-Jakob Disease Diagnostics.- Application of Single Molecule Array (SIMOA) in Cerebrospinal Fluid.- Luminex Multiplex Immunoassay for Proteomic Profiling of Cerebrospinal Fluid.- Monitoring Cerebrospinal Fluid Inflammatory Mediators by Olink Target 48 Cytokine Panel.- Targeted Absolute Quantification of Protein Biomarkers in Cerebrospinal Fluid by FastCAT.- Cerebrospinal Fluid Phosphoproteome Characterization Using Titanium Dioxide Enrichment and High-Resolution LC-MS/MS.- Analyzing N-Glycans in Cerebrospinal Fluid: A Glycomics Approach.- Application of Isotope Dilution Gas Chromatography-Mass Spectrometry Using Selected Ion-Monitoring to Evaluate Nonenzymatic Protein Damage in Cerebrospinal Fluid.- Phosphoproteomics of Cerebrospinal Fluid-Derived Extracellular Vesicles Applied to Primary Central Nervous System Lymphoma.- High-Throughput Human Cerebrospinal Fluid Proteome Analysis with Direct Data-Independent Acquisition (dDIA).- Olink® Explore for High-Throughput Protein Biomarker Discovery in Cerebrospinal Fluid.- High Throughput Proteomics Using the SomaLogic Platform.- A Comprehensive Workflow for Cerebrospinal Fluid Proteomics in the Study of Brain Tumors.- Mass Spectrometry Applied to Human Cerebrospinal Fluid Lipidome (II).- Analysis of Conformational Molecular Changes in Cerebrospinal Fluid Using Fourier Transform Infrared Spectroscopy in Neurodegenerative Diseases.- Elucidation of the Human Cerebrospinal Fluid Metabolome by Ion Mobility Mass Spectrometry and NMR Spectroscopy.- An LC-MS/MS Multiplexed Method to Quantify 14 Sphingolipids in Human Cerebrospinal Fluid (CSF).- Unraveling Potential Tauopathy Biomarkers in Cerebrospinal Fluid: Insights from Olfactory Tract Data.- Inspecting Biological Deregulation, Putative Markers, and Therapeutic Targets for Neurodegenerative Diseases through an Integrative Bioinformatics Analysis of the Human Cerebrospinal Fluid Proteome: A Tutorial.- Cerebrospinal Fluid Metabolomics and Proteomics Integration in Neurological Syndromes.- Drug Repositioning Based on Cerebrospinal Fluid Proteomes Using Connectivity Map Framework.
