Full Description
This second edition volume expands on the previous edition with an update on the broad spectrum of research models, techniques, and protocols used in laboratories by basic and clinical researchers. The chapters in this book are divided into two parts. Part One discusses the latest findings on the development and characterization of representative research models for chronic immune-based diseases and inflammation-associated cancers. Part Two covers biochemical, molecular, and cellular biological techniques that are commonly used to dissect the molecular mechanisms and cellular processes that drive the pathogenesis of certain disease states. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Inflammation and Cancer: Methods and Protocols, Second Edition is a valuable resource for those with a diverse range of laboratory-based experience, ranging from novice undergraduate students to established basic or clinical researchers who wish to diversify their existing portfolio of practical knowledge in the field.
Contents
Identifying Adult Stomach Tissue Stem/Progenitor Cells using the Iqgap3-2A-CreERT2 Mouse.- In Vitro and In Vivo Models for Metastatic Intestinal Tumors using Genotype-Defined Organoids.- On Target: An Intrapulmonary Transplantation Method for Modelling Lung Tumor Development in Its Native Microenvironment.- Endoscopic Ultrasound-Guided Fine Needle Biopsies to Generate Preclinical Disease Models to Study Inflammation in Pancreatic Ductal Adenocarcinoma.- Modeling Intestinal Carcinogenesis using In Vitro Organoid Cultures.- An In Vitro Model for Assessing Acute Lung Injury during Pancreatitis Development using Primary Mouse Cell Co-Cultures.- Tracking the Host Response to Infection in Peritoneal Models of Acute Resolving Inflammation.- Assessing Lung Inflammation and Pathology in Preclinical Models of Chronic Obstructive Pulmonary Disease.- Preclinical Mouse Model of Silicosis.- Tracking Cardiovascular Comorbidity in Models of Chronic Inflammatory Disease.- Live Imaging of Pyroptosis in Primary Murine Macrophages.- A Streamlined Method for Detecting Inflammasome-Induced ASC Oligomerization using Chemical Crosslinking.- DNA Methylation Analysis.- Flow Cytometry Identification of Cell Compartments in the Murine Brain.- Expression and Purification of Inflammasome Sensor Nod-Like Receptor Protein-1 using the Baculovirus-Insect Cell Expression System.- Dissecting lnterleukin-6 Classic- and Trans-Signaling in Inflammation and Cancer.- Selecting Therapeutic Antisense Oligonucleotides with Gene Targeting and TLR8 Potentiating Bifunctionality.- Exploring Allosteric Inhibitors of Protein Tyrosine Phosphatases through High-Throughput Screening.- Intravital Imaging of Regulatory T Cells in Inflamed Skin.- Confocal Endomicroscopy Monitoring of Tumor Formation.- Detection of Free Bioactive IL-18 and IL-18BP in Inflammatory Disorders.- MAC-seq: Coupling Low-Cost, High-Throughput RNA-seq with Image-Based Phenotypic Screening in 2D and 3D Cell Models.- Primary Intestinal Fibroblasts: Isolation, Cultivation, and Maintenance.- Lipid Nanoparticle-Mediated Delivery of miRNA Mimics to Myeloid Cells.- Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation.
