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Full Description
This thorough volume presents a wide range of existing methods, from the very popular to the more exotic, in the area of plant genotyping. Many methods of plant genotyping were initially developed for medical research, but all genotyping methods, if they are to be successful, should be suitable for application across the full range of studies within plant biology, as seen in this collection. Plant genotyping methods herein are based on a variety of assessments, including DNA microarray, with its hundreds of thousands of simultaneous reactions, or separate individual studies of DNA sequencing and fragment analysis, PCR and qPCR, allele-specific molecular probes and primers, digestion with restriction endonucleases, microscopy and many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Genotyping: Methods and Protocols offers researchers the opportunity to update their knowledge and choose the most suitable method of plant genotyping for their chosen application.
Contents
Genotyping by Sequencing (GBS) for Genome-Wide SNP Identification in Plants.- Genotyping by Multiplexed Sequencing (GMS) Using SNP Markers.- Computational Protocol for DNA Methylation Profiling in Plants Using Restriction Enzyme-Based Genome Reduction.- Double Digest Restriction-Site Associated DNA Sequencing (ddRADseq) Technology.- Whole Genome Wide SSR Markers Identification Based on ddRAD-Seq Data.- High-Throughput Association Mapping in Brassica napus L.: Methods and Applications.- Polyploid SNP Genotyping Using the MassARRAY System.- qPCR Genotyping of Polyploid Species.- Genome-Wide Association Studies (GWAS).- Transcriptomic Approach for Global Distribution of SNP/Indel and Plant Genotyping.- Specific-Locus Amplified Fragment Sequencing (SLAF-Seq).- Modifications of Kompetitive Allele-Specific PCR (KASP) Genotyping for Detection of Rare Alleles.- Amplifluor-Based SNP Genotyping.- SNP Genotyping with Amplifluor-Like Method.- Semi-Thermal Asymmetric Reverse PCR (STARP) Genotyping.- Modified Allele-Specific qPCR (ASQ) Genotyping.- Allele-Specific Mutation Genotyping with Mismatches in Primer Design.- PCR Allele Competitive Extension (PACE).- Molecular Beacon Probe (MBP)-Based Real Time PCR.- Molecular Beacons - Loop-Mediated Amplification (MB-LAMP).- TaqMan Probes for Plant Species Identification and Quantification in Food and Feed Traceability.- Tetra-Primer Amplification Refractory Mutation System (T-ARMS).- Penta-Primer Amplification Refractory Mutation System (PARMS) with Direct PCR-Based SNP Marker-Assisted Selection (D-MAS).- High-Resolution Melting (HRM) Genotyping.- Modified High-Resolution Melting (HRM) Marker Systems Increasing Discriminability Between Homozygous Alleles.- A New SNP Genotyping Technology by Target SNP-Seq.- Derived Polymorphic Amplified Cleaved Sequence (dPACS) Assay.- Tubulin-Based Polymorphism (TBP) in Plant Genotyping.- Multiplexed ISSR Genotyping by Sequencing (MIG-Seq).- Application of SolCAP Genotypingin Potato (Solanum tuberosum l.) Association Mapping.- Fluorescence In Situ Hybridization (FISH) for Genotyping of Triticeae Tribe Species and Hybrids.- Innovative Advances in Plant Genotyping.
