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Full Description
This fully updated book presents an account of areas of utility, techniques, and bioinformatic advancements in the field of lipidomics. Beginning with protocols for lipid isolation and extraction, the volume continues with techniques from extractive mass spectrometry to imaging mass spectrometry methods allowing localization of lipids in tissues. These protocols have been complemented by methods addressing specific problems from membranes, fractionated subcellular compartments or organelles to whole organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Lipidomics: Methods and Protocols, Second Edition serves as an ideal guide for biochemists, molecular biologists, neuroscientists, vision research scientists, as well as all biomedical researchers with interest in disease discovery and drug development.
Contents
Isolation of Mitochondrial Lipids and Mass Spectrometric Analysis.- Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation.- Multi-Macromolecular Extraction from Endosymbiotic Anthozoans.- High Resolution Liquid Chromatography-Mass Spectrometry for Lipidomics.- Characterization of Gangliosides from Mouse Optic Nerve Samples Using Mass Spectrometry.- Protocol and Methods Applicable to Retinal Vascular Diseases.- Analysis and Annotation of Phospholipids by Mass Spectrometry-Based Metabolomics.- Profiling the Mammalian Lipidome by Quantitative Shotgun Lipidomics.- Lipidomics Analysis with Triple Quadrupole Mass Spectrometry.- High-Performance Chromatographic Separation of Cerebrosides.- Quantification of Endocannabinoids from Biological Samples Using Liquid Chromatography Tandem Mass Spectrometry.- Analysis of Lipids, Fatty Acid, and Cholesterol in Membrane Microdomains.- Analysis of Cholesterol Lipids Using Gas Chromatography Mass Spectrometry.- Analyses and Localization of Phosphatidylcholine and Phosphatidylserine in Murine Ocular Tissue Using Imaging Mass Spectrometry.- A Robust Phenotypic Screening Assay Utilizing Human Podocytes to Identify Agents that Modulate Lipid Droplets.- Capillary Electrophoresis Plasma Fractionation for Lipids Analysis.- Combined Use of MALDI-TOF Mass Spectrometry and 31P NMR Spectroscopy for the Analysis of (Phospho)Lipids.- Single Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles of Pseudomonas aeruginosa.- Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells.- Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate.- Lipid Extraction and Sample Preservation Techniques for Stable Isotope Analysis and Ecological Assays.- Rapid Analysis of Plasmalogen Individual Species by High-Resolution Mass Spectrometry.- Untargeted Analysis of Lipids Containing Very Long Chain Fatty Acids in Retina and Retinal Tight Junctions.- Analysis of Lipid Contents in Human Trabecular Meshwork Cells by Multiple Reaction Monitoring (MRM) Profiling Lipidomics.- Mass Spectrometry Approaches for Detection and Determination of Prostaglandins from Biological Samples.- Downloading and Analysis of Metabolomic and Lipidomic Data from Metabolomics Workbench Using MetaboAnalyst 5.0.- Accurate Analysis of Lipid Building Blocks Using the Tool LipidOne.- Image Based Longitudinal Characterization of Corneal Wound to Understand the Role of Sphingosine-1-Phosphate.- Characterization of Trabecular Meshwork Mechanical Property Modulation after Application of Lipids.- C-Laurdan: Membrane Order Visualization of HEK293t Cells by Confocal Microscopy.- Sonication-Based Basic Protocol for Liposome Synthesis.
