Full Description
This volume provides researchers with the most updated understanding of the basics of West Nile Virus (WNV). Chapters focus on the biology, virology, epidemiology, pathogenesis, as well as the step-by-step molecular, cellular, and statistical methods to study WNV infection in cell culture, mosquitos, animal models, and human clinical specimen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, West Nile Virus: Methods and Protocols aims to be a valuable resource for all researchers interested in learning more about this important and developing field.
Contents
Introduction to West Nile Virus.- Quantification of West Nile virus by Plaque-Forming Assay.- Viral titer quantification of West Nile virus by immunostaining plaque assay.- Isolate and Culture Mouse Primary Neurons for West Nile Virus Infection.- Isolation of Murine Bone Marrow Derived Neutrophils for Infection Modeling.- Methods to study West Nile Virus infection, and the virus-induced inflammation in the brain in a murine model.- Detection of West Nile virus envelope protein in brain tissue with an immunohistochemical assay.- Quantitative Analysis of B Cell Subpopulations in Bone Marrow by Flow Cytometry.- Isolation of exosomes or extracellular vesicles from West Nile Virus-infected N2a cells, Primary Cortical Neurons and Brain Tissues.- Protocol to Study West Nile Virus Infection in Brain Slices In Vitro.- Studying Virus-Host Interactions with CRISPR technology.- Protocol of detection of West Nile Virus in Clinical Samples.- Detection and Analysis of West Nile Virus Structural Protein Genes in Animal Or Bird Samples.- Mosquito surveillance for West Nile Virus.- Molecular surveillance of West Nile virus in mosquitoes and sentinel chickens.- Statistical tools for West Nile Virus Disease Analysis.- Analytical Approaches to Uncover Genetic Associations for Rare Outcomes: Lessons from West Nile Neuroinvasive Disease.- Safety procedures to work with West Nile virus in biosafety level 3 facilities.- Development of antibody-based therapeutics against West Nile virus in plants.
