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Full Description
This volume focuses on protein analysis, and covers a wide array of uses of protein microarray for disease analysis. The chapters in this book discuss different stages of protein microarrays from their construction to their use, including different types of protein microarrays such as recombinant proteins, antibody, phage, and NAPPA protein microarrays, in planar format or in solution via beads arrays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Protein Microarrays for Disease Analysis: Methods and Protocols is a valuable resource for graduate and post-doctoral fellows interested in protein microarrays, as well as senior researchers interested in gaining more insight into this developing field.
Contents
Protein Microarrays Based Proteomics for Disease Analysis.- Systematic and Rational Design of Protein Arrays in Non-Contact Printers: Pipeline and Critical Aspects.- Phage Microarrays for Screening of Humoral Immune Responses.- Identification of Antibody Biomarker using High-Density Nucleic Acid Programmable Protein Array.- Bead-Based Assays for Validating Proteomic Profiles in Body Fluids.- Analysis of Protein-Protein Interactions by Protein Microarrays.- Detection of Post-Translational Modification Autoantibodies using Peptide Microarray.- Epitope Mapping of Allergenic Lipid Transfer Proteins.- Epitope Mapping of Food Allergens Using Non-Contact Piezoelectric Microarray Printer.- Protein Arrays for the Identification of Seroreactive Protein Markers for Infectious Diseases.- Glass Slide-Printed Protein Arrays as a Platform to Discover Serodiagnostic Antigens against Bacterial Infections.- Affinity Proteomics Assays for Cardiovascular and Atherosclerotic Disease Biomarkers.- Nucleic Acid Programmable Protein Arrays (NAPPA) for the Discovery of Autoantibodies in Osteoarthritis.- Profiling Autoantibody Responses to Devise Novel Diagnostic and Prognostic Markers using High-Density Protein Microarrays.- Deciphering Intracellular Signaling Pathways in Tumoral Pathologies.- Combination of Antibody Arrays to Functionally Characterize Dark Proteins in Human Olfactory Neuroepithelial Cells.- Protein Microarrays for Ocular Diseases.- Statistical Methods for Analysis of Protein Microarray Data using R.
