Elisa and Other Solid Phase Immunoassays : Techniques and Theory

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Elisa and Other Solid Phase Immunoassays : Techniques and Theory

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  • 製本 Paperback:紙装版/ペーパーバック版
  • 言語 ENG
  • 商品コード 9780471909828
  • DDC分類 500

Full Description


ELISA and Other Solid Phase Immunoassays Theoretical and Practical Aspects D. M. Kemeny, Department of Medicine, and S. J. Challacombe, Department of Oral Medicine and Pathology, United Medical and Dental Schools of Guy's and St Thomas's Hospitals, London, UK This guide to the modern applications of solid phase immunoassays is written by experts from a wide range of specialities who have used ELISA and other assays in a variety of fields. Not only is the book ideally suited for use as a laboratory manual but, recognizing the importance of understanding the theoretical basis of such assays, the authors also provide a comprehensive account of the scientific principles involved. The book analyses the solid phase supports used, as well as amplification systems, quantification and affinity of antibodies, detection systems, and the application of assays to cell biology, immunology and microbiology. Laboratory workers in the fields of medicine, dentistry, biochemistry, immunology, food science, and forensic and veterinary medicine will find this an invaluable source of advice, guidance and information on solid phase immunoassays.Other titles of interest from Wiley include two books edited by Professor W. P. Collins - Alternative Immunoassays and Complementary Immunoassays - as well as the following: Allergy: An International Textbook Edited by M. H. Lessof, T. H. Lee and D. M. Kemeny

Table of Contents

BASIC ASPECTS.
An Introduction to ELISA (D. Kemeny & S.
Chanter).
Microtitre Plates and Other Solid Phase
Supports (D. Kemeny & S. Challacombe).
Quantitative Aspects of Solid Phase
Immunoassays (R. Hamilton & N. Atkinson).
AMPLIFICATION.
Amplification by Second Enzymes (A.
Johannsson & D. Bates).
The Amplified ELISA (a-ELISA):
Immunochemistry and Applications (J. Butler).
MOLECULAR ASPECTS.
The Role of Antibody Affinity in the
Performance of Solid Phase Assays (M. Devey &
M. Steward).
The Immunochemistry of Sandwich ELISAs:
Principles and Applications for the
Quantitative Determination of Immunoglobulins
(J. Butler).
The Use of ELISA in the Characterization of
Protein Antigen Structure and Immune Response
(A. Pesce & J. Michael).
The Modified Sandwich ELISA (SELISA) for the
Detection of IgE and Other Antibody Isotypes
(D. Kemeny).
CELLULAR ELISAs.
The Solid Phase Enzyme-Linked Immunospot
Assay (ELISPOT) for Enumerating
Antibody-Secreting Cells: Methodology and
Applications (C. Czerkinsky, et al.).
ELISA-Plaque Assay for the Detection of
Single Antibody-Secreting Cells (J. Sedgwick
& P. Holt).
CHEMILUMINESCENCE.
Chemiluminescence Immunoassay (I. Weeks & J.
Woodhead).
APPLICATIONS TO MICROBIOLOGY.
The Use of ELISA for Rapid Viral Diagnosis:
Viral Antigen Detection in Clinical Specimens
(S. Chantler & A. Clayton).
The Use of ELISA for Rapid Viral Diagnosis:
Antibody Detection (R. Duncan).
Application of ELISA to Bacteriology (S.
Challacombe).
Appendix.
Index.