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Full Description
Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested protocols, outlining the principles upon which each technique is founded, as well as providing instructions on equipment setup and use, buffer preparation, reagents required, safety considerations and analysis of findings. Beginning with an introduction to biochemistry and laboratory procedures, the book moves on to specific methods focused on investigation of carbohydrates, proteins, enzymes, plant hormones, minerals, amino acids, and more.
The large range of protocols covered in this foundational, how-to reference are interdisciplinary and adaptable to a variety of areas, making this an ideal resource for researchers across various fields, including biochemistry, molecular biology, medical sciences, plant physiology, agriculture, and related subjects.
Contents
1. Introduction to
2. Introduction to laboratory procedures
3. Preparation of solutions and buffers
4. Qualitative analysis of carbohydrates
5. Qualitative analysis of proteins
6. pH (negative logarithm of hydrogen ion concentration)
7. Spectrophotometry
8. Carbohydrates
9. Amino acids and proteins
10. Enzyme assays
11. Vitamin assays
12. Phenolics
13. Plant pigments
14. Plant
15. Antinutritional factors in plants
16. Toxins
17. Extraction and identification of polyacetylenes
18. Alkaloids
19. Plant hormones
20. Immunochemical methods
21. Nitrogenase activity
22. Chlorophyll
23. Proximate analysis
24. Minerals
25. Antinutritional factors in plants
26. Antioxidants
27. Lipids
