Description
This second edition volume provides an updated and comprehensive overview of miRNA target identification, spanning miRNA binding site prediction, integrative and network-based analyses, emerging technologies, and experimental validation strategies. Expanding on the first edition, this volume includes updated versions of several core chapters, as well as entirely new chapters that reflect major conceptual and technological advances in the field. The chapters in this book cover topics such as principles and evolving features of miRNA target recognition and prediction; systems-level and integrative analyses of miRNAs and their targets; emerging technologies and translational applications; and experimental validation of predicted miRNA targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, MicroRNA Target Identification: Methods and Protocols, Second Edition is a valuable resource for researchers working in computational biology, molecular biology, and translational medicine. This book inspires the continued evolution of the field and the increasing integration of computational and experimental perspectives in miRNA research.
Table of Contents
miRNA Target Prediction: An Overview of the Past and Current Tools.- Target-Directed miRNA Degradation: Mechanisms and Significance.- Sfold Tools for MicroRNA Target Prediction.- Predicting miRNA Targets at the isomiR Level.- isoTar: miRNA-Target Consensus Prediction with Enrichment Analysis for Canonical and Modified miRNAs.- MeREDiBase: A Comprehensive Resource for miRNA Editing Events.- Construction and Analysis of miRNA Regulatory Networks.- Computational Methods for Pathway and Network Analysis with MicroRNAs.- Computational Resources for Studying miRNA-IncRNA Interactions.- Construction and Analysis of circRNA-miRNA-mRNA Regulatory Networks.- Identification of Disease-miRNA Networks Across Different Human Complex Diseases Using SWIM and MIENTURNET Tools.- Integration of Omics Data to Identify Cancer-Related MicroRNA.- MicroRNAs and Their Profiling via Single-Cell Sequencing Technologies.- The Yenos/MinION Multi-Cancer Early Detection Liquid Biopsy Test: An Innovative Approach to MicroRNA Quantification.- How to Validate miRNA Targeting: Western Blot Analysis.- Luciferase Reporter Assay for MicroRNA Target Validation.- Experimental Validation of MicroRNA Targets: Mutagenesis of Binding Regions Mutagenesis-Based Validation of MicroRNA Target Binding.
