Description
Algal Culturing Techniques is a comprehensive reference on all aspects of the isolation and cultivation of marine and freshwater algae, including seaweeds. It is divided into seven parts that cover history, media preparation, isolation and purification techniques, mass culturing techniques, cell counting and growth measurement techniques, and reviews on topics and applications of algal culture techniques for environmental investigations.Algal Culturing Techniques was developed to serve as both a new textbook and key reference for phycologists and others studying aquatic systems, aquaculture and environmental sciences. Students of algal ecology, marine botany, marine phycology, and microbial ecology will enjoy the hands-on methodology for culturing a variety of algae from fresh and marine waters. Researchers in industry, such as aquaculture, pharmaceutical, foodstuffs, and biotechnology companies will find an authoritative and comprehensive reference.- Sponsored by the Phycological Society of America- Features color photographs and illustrations throughout- Describes culturing methods ranging from the test tube to outdoor ponds and coastal seaweed farms- Details isolation techniques ranging from traditional micropipette to automated flow cytometeric methods- Includes purification, growth, maintenance, and cryopreservation techniques- Highlights methods for estimating algal populations, growth rates, isolating and measuring algal pigments, and detecting and culturing algal viruses- Features a comprehensive appendix of nearly 50 algal culture medium recipes- Includes a glossary of phycological terms
Table of Contents
1. Historical Review of Algal Culturing Techniques2. Freshwater Culture Media3. Marine Culture Media4. Trace Metal Ion Buffers and Their Use in Culture Studies5. Sterilization and Sterile Technique6. Traditional Microalgae Isolation Techniques7. Automated Isolation Techniques for Microalgae8. Purification Methods for Microalgae9. Isolation and Purification Techniques for Macroalgae10. Perpetual Maintenance of Actively Metabolizing Microalgal Cultures11. Long Term Macroalgal Culture Maintenance12. Cryopreservation Methods for Maintaining Microalgal Cultures13. Photobioreactors and Fermentors: The Light and Dark Sides of Growing Algae14. Culturing Microalgae in Outdoor Ponds15. Mariculture of Seaweeds16. Counting Cells in Culture Using the Light Microscope17. Phytoplankton Cell Counting by Flow Cytometry18. Measuring Growth Rates in Microalgal Cultures19. Using Cultures to Investigate the Physiological Ecology of Microalgae20. Analysis of Algal Pigments by High-Performance Liquid Chromatography21. Endogenous Rhythms and Daylength Effects in Macroalgal Development22. Viral Contamination of Algal Cultures23. Control of Sexual Reproduction in Algae in Culture24. Microalgal Life Cycle Dynamics: Encystment and Excystment25. Cultures as a Means of Protecting Biological Resources: Ex-situ Conservation of Threatened Algal Species
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