Radiation Cytogenetics : Methods and Protocols

著者名：Kato, Takamitsu A. (EDT)/Wilson, Paul F. (EDT)

Humana（2019/07/02発売）

• 言語：ENG
• ISBN：9781493994304
• eISBN：9781493994328

Description

This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices workingon their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory.

Table of Contents

Human Lymphocyte Metaphase Chromosome Preparation for Radiation-Induced Chromosome Aberration Analysis.- Animal Lymphocyte Metaphase Chromosome Preparation.- Micronuclei Formation Analysis after Ionizing Radiation.- G1 Premature Chromosome Condensation (PCC) Assay.- G2 Chromosomal Radiosensitivity Assay for Testing Individual Radiation Sensitivity.- G2 Premature Chromosome Condensation/Chromosome Aberrations Assay: Drug-Induced Premature Chromosome Condensation (PCC) Protocols and Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin for Radiation Biology.- Sister Chromatid Exchange as a Genotoxic Stress Marker.- DNA Damage Focus Formation Assay.- Nuclear Foci Assays in Live Cells.- In Situ DNA Damaging Foci Analysis on Metaphase Chromosomes.- PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations.- Modified PNA Telomere and Centromere FISH Protocols.- Directional Genomic Hybridization (DGH) for Detection of Intra-Chromosomal Rearrangements.- Reciprocal Translocation Analysis with Whole Chromosome Painting for FISH.- Analysis of Radiation-Induced Chromosome Exchanges Using Combinatorial Chromosome Painting.