Description
Thisvolume summarizes current techniques; ranging from protocols for virus growth,isolation, quantification and generation of recombinant RSV virus to proceduresfor the efficient characterization of the host immune response to RSVinfection. These techniques are used in numerous laboratories around the worldand are the building blocks that support the majority of RSV virus research. Written in the highly successful Methods in MolecularBiology series format, chapters include introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.
Authoritative and cutting-edge, Humana Respiratory Syncytial Virus:Methods and Protocols aims to ensure successful resultsin the further study of this vital field.
Table of Contents
Human Respiratory Syncytial Virus: An Introduction.- RSV Growth and Quantification by Microtitration and qRT-PCR Assays.- Quantificationof RSV Infectious Particles by Plaque Assay and Immunostaining Assay.- Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays.- Secretory Expression and Purification of Respiratory Syncytial Virus G and FProteins in Human Cells.- Development of Human MonoclonalAntibodiesagainst Respiratory Syncytial Virus Using a High Efficiency HumanHybridoma Technique.- Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of ImmuneProtection.- Host Factors Modulating RSV Infection; Use of Small Interfering RNAs to ProbeFunctional Importance.- In Vitro Modelling of RSV Infection and Cytopathogenesis inWell-Differentiated Human Primary Airway Epithelial Cells (WD-PAECs).- Reverse Genetics of Respiratory Syncytial Virus.- Use of Minigenome Systems to Study RSVTranscription.- Screening for Host Factors DirectlyInteracting with RSV Protein: Microfluidics.- A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particlesto Identify Cellular Factors as Drug Targets.- MicroRNA Profiling from RSV Infected Biofluids, Whole Blood, and Tissue Samples.- Mouseand Cotton Rat Models of Human Respiratory Syncytial Virus.- In Vivo Assessment of Airway Function in the Mouse Model.- Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus.
