Advances in Protein Molecular and Structural Biology Methods

著者名：Tripathi PhD, Timir (EDT)/Dubey, Vikash Kumar (EDT)

Academic Press（2022/01/14発売）

• 言語：ENG
• ISBN：9780323902649
• eISBN：9780323902656

Description

Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering.It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application.- Presents a thorough overview of the latest and emerging methods and technologies for protein study- Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy- Includes computational and machine learning methods- Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins

Table of Contents

Section I: Techniques to optimize recombinant protein expression in bacteria, yeast, insect, and mammalian cells1. Strategies to Improve the Expression and Solubility of Recombinant Proteins in BacteriaNiharika Nag, Heena Khan, and Timir Tripathi2. Advances in Heterologous Protein Expression Strategies in Yeast and Insect SystemsMeenakshi Singh, Smita Gupta, Arun Kumar Rawat, Sudhir Kumar Singh3. Methods for Transient Expression and Purification of Monoclonal Antibodies in Mammalian CellsSuchitra Kamle, Dawei Li, Chun Geun Lee, Jack A. Elias4. Methods for Recombinant Production and Purification of Intrinsically Disordered ProteinsSteffen P. GraetherSection 2: Biophysical techniques including, NMR, X-ray crystallography, cryo-EM, etc.5. Methods to Determine the Oligomeric Structure of ProteinsPurna Bahadur Chetri, Heena Khan, and Timir Tripathi6. Multimodal Methods to Study Protein Aggregation and FibrillationMaria Georgina Herrera, Marco Giampà, Nicolo Tonali　and Veronica Isabel Dodero7. Experimental Methods to Study the Thermodynamics of Protein-Protein InteractionsSantanu Sasidharan, Niharika Nag, Timir Tripathi, and Prakash Saudagar8. Experimental Methods to Study the Kinetics of Protein-Protein InteractionsAbhay Narayan Singh, Kristijan Ramadan, Shalini Singh9. Computational Techniques to Study Protein-Protein Interactions Khattab Al-Khafaji, Tugba Taskin-Tok10. Experimental Methods to Study Protein-Nucleic Acid InteractionsRoberto Giambruno, Jakob Rupert, and Elsa Zacco11. Advanced Computational Tools for Quantitative Analysis of Protein-nucleic acid InterfacesSunandan Mukherjee and Chandran Nithin12. Experimental Techniques to Study Protein Dynamics and ConformationsAkshita Gupta, Anamika Singh, Nabeel Ahmad, Tej P. Singh, Sujata Sharma, Pradeep Sharma13. Computational Techniques to Study Protein Dynamics and Conformations Laura Orellana, Anil Mhashal, and Agusti Emperador14. Application of Circular Dichroism Spectroscopy in Studying Protein Folding, Stability, and InteractionMd. Anzarul Haque, Punit Kaur, Asimul Islam, and Md. Imtaiyaz Hassan15. Studying Protein Folding Dynamics using Single Molecule Fluorescence MethodsNarattam Mandal, Krishnananda Chattopadhyay, Achinta Sannigrahi16. Advances in Liquid-state NMR Spectroscopy to Study the Structure, Function, and Dynamics of BiomacromoleculesPriyanka Aggarwal, Pooja Kumari and Neel Sarovar Bhavesh17. In-Cell NMR Spectroscopy: A Tool for Cellular Structure BiologyVijay Kumar18. Current Trends in Membrane Protein CrystallographyKoomity V. Nageswar, Mansi Sharma, Dipak N. Patil, Santoshi Nayak, Anwesha Roy, Appu K. Singh19. Advances in Sample Preparation and Data Processing for Single-particle Cryo-Electron MicroscopyAnshul Assaiya, Suparna Bhar and Janesh Kumar20. Advanced Mass Spectrometry-Based Approaches for Molecular-Structural Biologists Joanna Bons, Jacob Rose, Amy O'Broin, and Birgit Schilling21. Developments, Advancements, and Contributions of Mass Spectrometry in Omics TechnologiesSaravanan Kumar22. Role of Structural Biology Methods in Drug DiscoveryFouzia Nasim and Insaf Ahmed QureshiSection 3: Computational methods including MD simulation, coupling MD and deep machine learning, etc23. Prediction, Validation, and Analysis of Protein Structures: A Beginner's GuideSantanu Sasidharan and Prakash Saudagar24. Advances in Structure-Based Virtual Screening for Drug DiscoveryOlujide O. Olubiyi, Suman Samantray and Alexander-Maurice Illig25. Methods and Applications of Machine Learning in Structure-Based Drug DiscoveryMadhumathi Sanjeevi, Prajna N Hebbar, Natarajan Aiswarya, Rashmi S., Chandrasekar Narayanan Rahul, Ajitha Mohan, Jeyaraman Jeyakanthan and Kanagaraj Sekar26. Molecular Dynamics Simulations: Principles, Methods, and Applications in Protein Conformational Dynamics Aditya K. Padhi, Matej Janezic, Kam Y. J. Zhang27. Applications of Molecular Dynamics Simulations in Drug DiscoveryXubo Lin28.