Description
Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps.
Key Features:
- Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience
- Includes chemical biology as its foundation, teaching readers experimental methods specific to the field
- Provides instructor experiments that are easy to prepare and execute, at comparatively low cost
- Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry
- Is written by an expert in the field
This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.
Table of Contents
Author Bio
Chapter 1 Buffers
Chapter 2 Assays
Chapter 3 Protein Concentration
Chapter 4 ELISA
Chapter 5 Salting Out Proteins and Other Biomolecules
Chapter 6 A Discussion of Isoelectric Point and Effective Charge
Chapter 7 Column Chromatography
Chapter 8 Michaelis–Menten Kinetics
Chapter 9 Protein Purification
Chapter 10 Polyacrylamide Gels
Chapter 11 In Silico Biochemistry: The Evolution of Globins
Chapter 12 Growing Crystals of Hemoglobin
Chapter 13 Enzyme Inhibition
Chapter 14 Multisubstrate Kinetics
Chapter 15 Fluorescence and Denaturation
Chapter 16 Fluorescence Studies of Ligand Binding
Chapter 17 DNA Restriction Digests
Chapter 18 Western Blotting
Index
