Description
Diagnostic Molecular Biology describes the fundamentals of molecular biology in a clear, concise manner to aid in the comprehension of this complex subject. Each technique described in this book is explained within its conceptual framework to enhance understanding.
The targeted approach covers the principles of molecular biology including the basic knowledge of nucleic acids, proteins, and genomes as well as the basic techniques and instrumentations that are often used in the field of molecular biology with detailed procedures and explanations. This book also covers the applications of the principles and techniques currently employed in the clinical laboratory.
- Provides an understanding of which techniques are used in diagnosis at the molecular level
- Explains the basic principles of molecular biology and their application in the clinical diagnosis of diseases
- Places protocols in context with practical applications
Table of Contents
Part I1. Nucleic Acids: DNA and RNA2. Nucleic Acid-Based Cellular Activities3. Gene Expression: Transcription of the Genetic Code4. Gene Expression: Translation of the Genetic Code5. The Genome
Part II6. Extraction and Purification of Nucleic Acids and Proteins7. Detection and Analysis of Nucleic Acids8. Quantification and Analysis of Proteins9. Amplification of Nucleic Acids10. Characterization of Nucleic Acids and Proteins11. Techniques in Sequencing12. Genome and Transcriptome Analysis
Part III13. Molecular Diagnosis of Chromosomal Disorders14. Molecular Diagnosis of Mutation and Inherited Diseases15. Molecular Diagnosis of Infectious Diseases16. Guidance for Molecular Clinical Laboratory
