Description
Recent advances in array-based detectors and imaging technologies have provided high throughput systems that can operate within a substantially reduced timeframe and other techniques that can detect multiple contaminants at one time. These technologies are revolutionary in terms of food safety assessment in manufacturing, and will also have a significant impact on areas such as public health and food defence. This book summarizes the latest research and applications of sensor technologies for online and high throughput screening of food.The book first introduces high throughput screening strategies and technology platforms, and discusses key issues in sample collection and preparation. The subsequent chapters are then grouped into four sections: Part I reviews biorecognition techniques; Part II covers the use of optical biosensors and hyperspectral imaging in food safety assessment; Part III focuses on electrochemical and mass-based transducers; and finally Part IV deals with the application of these safety assessment technologies in specific food products, including meat and poultry, seafood, fruits and vegetables.- Summarises the latest research on sensor technologies for online and high-throughput screening of food- Covers high-throughput screening and the current and forecast state of rapid contaminant detection technologies- Looks at the use of optical and electrochemical biosensors and hyperspectral imaging in food safety assessment and the application of these technologies in specific food products
Table of Contents
- List of contributors- Woodhead Publishing Series in Food Science, Technology and Nutrition- 1. High throughput screening strategies and technology platforms for detection of pathogens: an introduction- Abstract- 1.1 Introduction- 1.2 Current detection strategies- 1.3 Why high throughput screening (HTS) is needed- 1.4 HTS technologies for foodborne pathogens – present and future trends- 2. Sampling and sample preparation for sensor-based detection of pathogens in foods- Abstract- 2.1 Introduction- 2.2 Key issues in sample preparation: from "Farm to Fork to Physician- 2.3 Challenges in sampling from food matrices and on "bulk surfaces- 2.4 Nonspecific vs. specific methods- 2.5 Physical methods- 2.6 Chemical and combined methods- 2.7 Capture and concentration of whole microbial cells- 2.8 The use of cleaning materials in sampling- 2.9 Capture and concentration of pathogen DNA from complex food matrices- 2.10 Innovations in selective enrichment strategies- 2.11 Conclusions- Part One: Biorecognition techniques- 3. Antibodies, enzymes, and nucleic acid sensors for high throughput screening of microbes and toxins in food- Abstract- 3.1 Introduction- 3.2 Conventional methods for bacterial pathogen detection- 3.3 Rapid and advanced technologies- 3.4 Antibody structure and production- 3.5 Polyclonal and monoclonal antibodies for biorecognition- 3.6 The identification of recombinant antibodies by phage display technology- 3.7 Biopanning of phage display libraries- 3.8 Biosensors and antibody immobilization strategies- 3.9 Immunosensor-based applications for high throughput pathogen screening- 3.10 Multiplexed pathogen detection using antibodies for biorecognition- 3.11 Nucleic acid assays- 3.12 Microarray-based technologies- 3.13 Enzyme-based sensors- 3.14 High throughput bacterial toxin detection- 3.15 High throughput fungal pathogen and mycotoxin detection- 3.16 Marine toxins- 3.17 Selected commercial platforms for high throughput detection- 3.18 Conclusion- 4. Phage technology in high throughput screening for pathogen detection in food- Abstract- Acknowledgments- 4.1 Introduction- 4.2 Pathogen detection using phage: culture-based methods and phage typing- 4.3 Pathogen detection using phage: phage-host adhesion-based methods- 4.4 Pathogen detection using phage: biosensors- 4.5 Pathogen detection using phage: phage-triggered ion cascade- 4.6 Pathogen detection using phage: phage replication and metabolism-based methods- 4.7 Pathogen detection using phage: phage lysis-based methods- 4.8 Conclusion- 5. Mammalian cell-based sensors for high throughput screening for detecting chemical residues, pathogens, and toxins in food- Abstract- Acknowledgments- 5.1 Introduction- 5.2 The need for novel methods in food control- 5.3 Cell-based biosensors for food safety- 5.4 Mammalian cell-based biosensors- 5.5 Robustness and shelf life of mammalian cell-based biosensors- 5.6 Conclusions and future trends- Part Two: Optical transducers and hyperspectral imaging- 6. Label-free light-scattering sensors for high throughput screening of microbes in food- Abstract- Acknowledgments- 6.1 Introduction- 6.2 Elastic light-scattering-based high throughput screening of microorganisms- 6.3 Application of BARDOT-based high throughput screening in food safety- 6.4 Future trends- 7. Vibrational spectroscopy for food quality and safety screening- Abstract- Acknowledgments- 7.1 Introduction- 7.2 Basic concepts of vibrational spectroscopy- 7.3 Applications in food quality- 7.4 Applications in food safety- 7.5 Hyperspectral imaging for food quality and safety- 7.6 Summary and future trends- 8. Flow cytometry and pathogen screening in foods- Abstract- Acknowledgments- 8.1 Introduction- 8.2 Analysis of foods using classical flow cytometry- 8.3 Analysis of foods using bead-based detection- 8.
