Description
DNA typing has revolutionized criminal investigations and has become a powerful tool in the identification of individuals in criminal and paternity cases. Forensic DNA Biology: A Laboratory Manual is comprised of up-to-date and practical experiments and step-by-step instructions on how to perform DNA analysis, including pipetting, microscopy and hair analysis, presumptive testing of body fluids and human DNA typing. Modern DNA typing techniques are provided, reflecting real life, where not all institutions and crime labs can afford the same equipment and software. Real case studies will be used throughout.- Provides practical step-by-step instruction on how to perform forensic DNA analysis- Includes analysis of hair, presumptive testing of body fluids, human DNA typing and statistics- Covers techniques such as pipetting, microscopy and DNA extraction- Pre- and post-lab exercises and questions assist the reader in learning the material- Report writing templates assure the reader learns real world crime lab procedure
Table of Contents
1. Pipetting2. Serology3. Sampling Biological Evidence for DNA Extraction4. DNA Extraction5. Determination of quality and quantity of DNA using agarose gel electrophoresis6. Determination of DNA quality and quantity using UV-vis spectroscopy7. Determination of DNA quantity by fluorescence spectroscopy8. Real-time polymerase chain reaction (PCR) quantitation of DNA9. Multiplex polymerase chain reaction (PCR) primer design (in silico)10. Testing designed PCR primers in multiplex reactions11. Multiplex polymerase chain reaction (PCR) amplification of short tandem repeat (STR) loci using a commercial kit12. Capillary electrophoresis of short-tandem repeat (STR) polymerase chain reaction (PCR) products from a commercial multiplex kit13. Computing random match probability from DNA profile data using population databases14. Mitochondrial deoxyribonucleic acid (mtDNA) single nucleotide polymorphism (SNP) detection15. Analysis of deoxyribonucleic acid (DNA) sequence data using bioedit16. Ribonucleic acid (RNA) extraction17. Y-STR PCR amplification18. Human genetic analysis: paternity or missing persons cases and statistics19. Low copy number stochastic results20. Using in silico methods to construct a short tandem repeat (STR) deoxyribonucleic acid (DNA) sequence for cloning21. Deoxyribonucleic acid (DNA) extraction from botanical material and polymerase chain reaction (PCR) amplification22. Social, ethical and regulatory concerns23. Selected forensic DNA biology case studiesAppendix: Allele frequencies of CODIS loci
