Description
Methods in Neurosciences, Volume 6: Neuropeptide Technology: Synthesis, Assay, Purification, and Processing describes procedures and tools of assay useful for the identification, purification, and quantification of neuropeptides and their receptors. This volume is divided into four sections— chemical synthesis and biosynthesis; measurement of neuropeptides; purification and characterization; and neuropeptide degrading and processing enzymes. In these sections, this book specifically discusses the synthesis of peptide substrates for protein kinase C; synthesis of glycosyl neuropeptides; and ultrastructural localization of peptides. The measurement of neurokinin B by radioimmunoassay; purification and characterization of neuroendocrine peptides from rat brain; and preparation of glia maturation factor ß are also elaborated. This text likewise covers the assays for arginine/lysine carboxypeptidases and enzymes that metabolize atrial natriuretic peptide. This publication is beneficial to neuroscientists and students researching on the synthesis, assay, purification, and processing of neuropeptides.
Table of Contents
Contributors to Volume 6PrefaceVolumes in SeriesSection I Chemical Synthesis and Biosynthesis 1. Large-Scale Synthesis of Gonadotropin-Releasing Hormone Antagonists for Clinical Investigations 2. Synthesis of Peptide Substrates for Protein Kinase C 3. Synthesis of Glycosyl Neuropeptides 4. Assays for Biosynthesis of Thyrotropin-Releasing Hormone 5. Microsequence Analysis of Proteins Purified by Gel Electrophoresis 6. Preparation of Site-Specific Peptide Immunogens Using Multiple Antigen Peptide Approach SystemSection II Measurement of Neuropeptides 7. Subcellular Distribution of Neuropeptides and Measurement of Their in Vitro Release 8. Analysis of Tachykinin Peptide Family Gene Expression Patterns by Combined High-Performance Liquid Chromatography-Radioimmunoassay 9. Ultrastructural Localization of Peptides: Comparison of Methods 10. Measurement of Immune System in Response to Peptides in Central Nervous System 11. Identification of Functionally Significant Phosphorylation Sites on Neuronal Proteins and Preparation of Antibodies That Recognize Them 12. Measurement of Brain Peptides: Angiotensin and Atrial Natriuretic Peptide in Tissue and Cell Culture 13. Regionally Specific Antisera to Human ß-Preprotachykinin 14. Measurement of Neurokinin B by Radioimmunoassay 15. Radioimmunoassay of Tachykinins 16. Radioimmunoassay of α-Melanocyte-Stimulating Hormone 17. Radioimmunoassay of Atrial Natriuretic Peptide 18. Radioimmunoassay of Cholecystokinin 19. Secretion and Radioimmunoassay of Somatostatin: In Vitro SystemSection III Purification and Characterization 20. Purification and Characterization of Immunoregulatory Peptides from Neuroendocrine Tissues: Suppressin as a Model 21. Purification and Characterization of Neuroendocrine Peptides from Rat Brain: Prosomatostatin Isolation 22. Preparation of Glia Maturation Factor ß 23. Detection and Purification of Thyrotropin-Releasing Hormone Precursor Peptides Using Antisera Generated against Synthetic Peptides 24. Physiologic Roles of Peptides as Central Nervous System TransmittersSection IV Neuropeptide Degrading and Processing Enzymes 25. Assays for Arginine/Lysine Carboxypeptidases: Carboxypeptidases H (E; Enkephalin Convertase), M, and N 26. Assay of Peptidase That Removes Carboxy-Terminal Tripeptide from 125I-Labeled Atrial Natriuretic Factor 27. Assays for Enzymes That Metabolize Atrial Natriuretic PeptideIndex
