Description
The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research.Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation.The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip.This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry.Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book.David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing.- Provides a unique mix of theory, practical advice and applications, with numerous examples- Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers- Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee- Provides valuable chapter updates, now available on www.immunoassayhandbook.com
Table of Contents
Part 1 IMMUNOASSAY FUNDAMENTALS1.1 How to use this book1.2 Immunoassays for beginners 1.3 Immunoassay performance measures Part 2 IMMUNOASSAY CONFIGURATIONS2.1 Competitive and immunometric assays (including ELISA)2.2 Non-competitive immunoassays for small molecules2.3 Homogenous immunoassays2.4 Lateral flow immunoassay devices2.5 Ambient analyte immunoassay2.6 Free analyte immunoassay2.7 Qualitative immunoassays2.8 Immunoassays for the detection or quantification of antibodies2.9 Microsphere-based Multiplex Immunoassays: Development and Applications using Luminex xMAP Technology2.10 Lab on a chip2.11 Immunological Biosensors2.12 SPR2.13 Single Molecule Array Part 3 IMMUNOASSAY COMPONENTS3.1 Antibodies3.2 Signal generation systems3.3 Solid-phase and other separation systems3.4 Conjugation methods3.5 Standardisation and calibration3.6 Calibration curve-fitting Part 4 RELATED TECHNIQUES4.1 Foundations of immunochemistry4.2 Immunohistochemistry and immunofluorescence Part 5 IMMUNOASSAY DEVELOPMENT5.1 Practical guide to ELISA development5.2 Performance evaluation and validation5.3 Interferences in immunoassay5.4 Immunoassay development in industry Part 6 IMMUNOASSAY IMPLEMENTATION6.1 Subject preparation, sample collection and handling6.2 Laboratory quality assurance6.3 Point-of-care testing6.4 Choosing an automated immunoassay system6.5 Troubleshooting guide Part 7 IMMUNOASSAY PRODUCT TECHNOLOGY7.1 Introduction to product technology7.2 Market trends7.3 Lateral Flow and Consumer Diagnostics 7.4 Clearblue7.5 TRIAGE7.6 Stratus CS7.7 IMx7.8 AxSYM7.9 Architect7.10 Advia CENTAUR7.11 IMMULITE and IMMULITE 10007.12 IMMULITE 2000 and IMMULITE 2000 Xpi7.13 Eci7.14 Vitros Clin chem7.15 Access 27.16 Dxl 600 and 8007.17 Elecsys7.18 PRISM7.19 Phadia systems Part 8 IMMUNOASSAY APPLICATIONS OTHER THAN CLINICAL CHEMISTRY8.1 Immunoassay applications in veterinary diagnostics8.2 Immunoassays in the pharmaceutical industry Part 9 IMMUNOASSAY CLINICAL APPLICATIONS (HUMAN)9.1 Clinical concepts9.2 Thyroid9.3 The adrenal cortex9.4 Bone metabolism9.5 Infertility9.6 In vitro fertilization and embryo transfer9.7 Hirsutism and virilization in the female9.8 Pregnancy9.9 Growth and growth hormone deficiency9.10 Diabetes mellitus9.11 Hematology9.12 Cardiac markers9.13 Cancer markers9.14 Allergy9.15 Autoimmune diseases9.16 GI Tract9.17 Hepatitis9.18 HIV9.19 Other viral diseases (excl HIV and Hepatitis)9.20 Other bacterial diseases9.21 Fungal and parasitic diseases9.22 Therapeutic drug monitoring9.23 Drugs of abuse
