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Full Description
This book provides a comprehensive reference on nucleic acid analysis. It systematically introduces the molecular biology foundations, hybridization technologies, and advanced nucleic acid detection methods, including polymerase chain reactions, CRISPR-based systems, and high-throughput sequencing. Additionally, it explores the biological functions, analysis methods, and applications of various nucleic acid biomarkers, including DNA single nucleotide polymorphism, DNA methylation, microRNA, mRNA splice variants, fusion gene transcripts, RNA methylation and COVID-19 RNA. This book is valuable for researchers, postgraduates and practitioners engaged in nucleic acid analysis research and technological development in colleges and universities, scientific research institutes, nucleic acid testing-related companies and hospitals.
Contents
Chapter 1. Polymerase Chain Reaction (PCR).- Chapter 2. Ligase Chain Reaction (LCR).- Chapter 3. Loop-mediated Isothermal Amplification (LAMP).- Chapter 4. Rolling Circle Amplification (RCA).- Chapter 5. Isothermal Exponential Amplification Reaction (EXPAR).- Chapter 6. Helicase- and Recombinase-Dependent DNA Isothermal Amplification (HDA and RPA).- Chapter 7. Nucleic Acid Detection Technologies Based on the CRISPR-Cas Gene Editing System.- Chapter 8. DNA Single Nucleotide Polymorphisms (SNP) Analysis.- Chapter 9. Biological Functions of DNA Methylation and Analytical Methods.- Chapter 10. Biological Functions and Analytical Methods of MiRNA.- Chapter 11. mRNA Biomarkers and Their Analytical Methods.- Chapter 12. Detection of SARS-CoV-2 Based on Nucleic Acid Analysis.
