Full Description
A knowledge of the practical aspects of clinical biochemistry is essential for medical students to understand the diagnostic and prognostic status of any ailment. This book addresses and compiles some of the invariably used protocols in biochemistry with the aim to enhance the understanding of the basics behind following the methodologies, including those for urine analysis; carbohydrate, protein, and lipid analyses; and diabetes and lipid profile. It discusses organ function tests, such as liver, renal, or pancreatic function tests, in addition to exploring pH and buffer, colorimeter, spectrophotometer, chromatography, electrophoresis, enzyme-linked adsorbent assay, radioimmunoassay, biosensor/glucometer, fructosamine, DNA isolation, mass spectrometry, flow cytometry, centrifugation, automation, cerebrospinal fluid examination, and oxalic acid test. The book also touches upon the basics of laboratory safety rules and laboratory glassware.
The book is helpful for students to understand the relationship between quality management and specimen collection and processing them for their biological functions. It is well-suited for academic courses, including undergraduate, graduate, postgraduate, and professional courses and covers the practical syllabus for MBBS, BDS, BSc MLT, BSc nursing, and MSc students. For undergraduate students in the disciplines of biochemistry, molecular biology, chemistry, and other related biological sciences, the book forms an integral part of the syllabus with its case studies, MCQs, biochemistry, OSPEs, etc. It also demonstrates the actual experiments for understanding the concepts better and is a must read for students to acquire skills to work with a clinical biochemistry laboratory.
Contents
1. Lab Safety Rules 2. Specimen Collection and Processing 3. Quality Management in the Laboratory 4. Urine analysis 5. Carbohydrates 6. Blood Glucose 7. Proteins 8. Lipid Analysis 9. Lipid Profile 10. Liver Function Test 11. Renal Function Test 12. Pancreatic Function Test 13. Acids and Bases 14. Colorimeter 15. Chromatography 16. Electrophoresis 17. Enzyme-Linked Adsorbent Assay 18. Radioimmunoassay 19. Polymerase Chain Reaction 20. Biosensors/Glucometer 21. Glycated Hemoglobin 22. Enzymes 23. DNA Isolation from Blood and Tissue 24. Mass Spectrometry 25. Flow Cytometry 26. Centrifugation 27. Automation 28. Cerebrospinal Fluid Examination 29. Oxalic acid
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