Full Description
The book discusses the various methods and protocols available in hairy root culture-based research. The utilization of Agrobacterium mediated genetic transformation and establishment of hairy root cultures has paved the way for large-scale secondary metabolite production in medicinal plants. Presenting recent research and offering insights from eminent research groups, the book covers a range of topics related to hairy root-based applications, including (i) establishment of hairy roots and native production of SM (ii) yield enhancement strategies for increased SM production, like elicitation (iii) hairy roots as a tool for value-added applications such as plant-microbe interaction, characterization of plant genes and root biology studies. As such it is an informative guide and experimental manual for researchers in diverse fields of plant biology.
Contents
Chapter 1. Hairy Roots Biotechnology Unzipped: A Journey of Reality and Promises.- Chapter 2. Hairy Roots as a Source of Tropane Alkaloids.- Chapter 3. Induction, Metabolite Analysis and Transgenesis of Hairy Roots From Coleus Forskohlii.- Chapter 4. Establishment of Hairy Roots of Endangered Himalayan Plantswertiachirata: A Sustainable Alternative to Extraction from Nature.- Chapter 5. A Protocol for the Selection of Spontaneous Variants From Established Catharanthusroseus Hairy Root Cultures.- Chapter 6. Strategies for the Optimization of Culture Conditions for Increasing Metabolite Production Through Hairy Root Cultures. Monoterpenoid Indole Alkaloid Production in Catharanthus Roseus Hairy Roots.- Chapter 7. Protocol for Enhanced Withaferin-a Production in Elicited Withania Somnifera (l.)Dunal Hairy Root Cultures.- Chapter 8. Establishment of Hairy Root Cultures of Pentalinonandrieuxii for the Production of Betulinic Acid.- Chapter 9. Tropane Alkaloid Production by the Establishment Of Hairy Root Cultures of Brugmansia Candida and Elicitation.- Chapter 10. Elicitation as an Essential Strategy For Enhancing Anthraquinone Accumulation In Hairy Root Cultures of Rubia Tinctorum.- Chapter 11. Establishment of Hairy Root Cultures for the Production of Biopharmaceuticals and Optimization of Methods for Recombinant Protein Secretion In The Culture Medium.- Chapter 12. An Improved Protocol for Agrobacterium Rhizogenes-Mediated Transformation of Recalcitrant Plants for Root Biology Studies: A Case Study of Tea Plants (Camellia Sinensis Var. Sinensis).- Chapter 13 Functional Analysis of Plant Genes Related to Arbuscular Mycorrhiza Symbiosis Using Agrobacterium Rhizogenes-Mediated Root Transformation and Hairy Root Production.- Chapter 14.Functional Characterization of Genes Involved in Legume Nodulation Using Hairy Root Cultures.- Chapter 15. Fluidigm Biomark Diagnostic Panel for Analysis of the Expression of Nicotiana Tabacum Genes, Associated with Alkaloid Synthesis.
