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Full Description
This manual, designed for undergraduate and postgraduate students in the field of biological sciences, provides step-by-step instructions for conducting various experiments in a molecular biology laboratory. In addition to being useful for students and researchers planning experiments in this field, the manual is also beneficial for those who wish to enhance their understanding of molecular diagnostics. The authors, who have extensive experience in molecular biology and are actively involved in research and teaching in the field, have included a set of objective questions at the end of each experiment to aid in comprehension. This manual will also be helpful for students preparing for competitive exams or regular theoretical exams.
Contents
Diagnosis of Drought Tolerance in Crop Plants by PEG Method
Identification of Salt Tolerant Crop Variety by Glycine Betaine (GB) Method
Total Protein Estimation by Folin-Lowry Method
Protein Electrophoresis Using SDS-PAGE
Enzyme Extraction and Assay Study of Polyphenol Oxidase (PPO; EC 1.14.18.1)
Enzyme Extraction and Assay Study of Catalase (EC 1.11.1.6)
Enzyme Extraction and Assay Study of Guaiacol Peroxidase (POX) (EC 1.11.1.7)
Enzyme Extraction and Activity Study of Superoxide Dismutase (EC 1.15.1.1)
Peroxidase Isozyme Study by Electrophoresis Method
DNA Isolation from Plant Sample and Quantification by Spectrophotometer
Quality Check of Plant Genomic DNA on Agarose Gel Electrophoresis
Randomly Amplified Polymorphic DNA (RAPD) and Electrophoresis
Southern Blotting Experiment
Total RNA Isolation from Plants and its Quantification by Spectrophotometer
Quality Check of Total RNA on Agarose Gel
cDNA Synthesis from Isolated Plant Total RNA
Indirect Competitive ELISA
Objective Questions
