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Full Description
This book titled Molecular, Enzymatic and Biochemical Assays for Agricultural Pests focuses on fundamental molecular techniques, encompassing DNA extraction and sequence submission to the NCBI database. Additionally, it delves into protein estimation, immunoblotting, and real-time PCR.
Moreover, the book features biochemical assays, such as glucose and protein triglycerides estimation, as well as stress-responsive enzymes. It is expected that researchers from diverse fields will find these techniques valuable for their own investigations.
Contents
Chapter 1. DNA Extractions from Insects Twinkle Sinha, Varun Arya and Srinivasa Narayana
Chapter 2. Amplification of the Mitochondrial Cytochrome Oxidase-1 (COX 1) Gene by the Polymerase Chain Reaction (PCR). Varun Arya1, Twinkle Sinha and Saniya Tyagi
Chapter 3. Separation and Visualization of DNA by Agarose Gel Electrophoresis Saniya Tyagi, Varun Arya and Vishrutha C
Chapter 4. Quantification of the Nucleic Acids Using the Nano-Drop Spectrophotometer and Submission of COI Sequences in NCBI Genbank Vishrutha C, Varun Arya and Srinivasa Narayana
Chapter 5. DNA Extraction, Colony PCR and Evaluation of Biochemical Properties of Culturable Gut Bacteria from Insects Vinay N and Srinivasa Narayana
Chapter 6. Immunoblotting Sriparna Pal and Rakesh Verma
Chapter 7. Real-Time or Quantitative Polymerase Chain Reaction Sriparna Pal and Rakesh Verma
Chapter 8. Estimation of Biochemical Parameters in Insects Parinita Singh, Priyanka Yadav, Arvind Kumar Patel,Sourabh Verma, Tamal Das, Pradeep Kumar, Simrata Dalpat Preeti Chhanda Sahoo and Bhupendra Kumar
Chapter 9. Determination of Enzymatic Antioxidants in Insects Parinita Singh, Priyanka Yadav, Arvind Kumar Patel, SourabhVerma, Tamal Das, Sikta Saroja, Paritosh Sharma, Devendra Kumar Mishra and Bhupendra Kumar
