Stepwise Culture of Human Adult Stem Cells (River Publishers Series in Biotechnology and Medical Research)

Rameshwar, Pranela (EDT)

River Publishers（2024/03発売）

• 製本 Hardcover:ハードカバー版／ページ数 144 p.
• 言語 ENG
• 商品コード 9788770228541
• DDC分類 616.02774

Full Description

This volume on stepwise techniques to culture adult human stem cells begins with the basic methods to culture adult stem cells followed by related methods to supplement stem cell culture. This and future volumes of techniques will allow laboratories across scientific entities - academic and commercial - to adapt the described methods for similar methods with cells from other species. The standardized techniques will allow across the board interpretation of other technologies such as Omics studies. These methods can be a starting platform for culturing stem cells even in laboratories that might not be familiar with the techniques.

Scientific and early clinical trials strongly favor the development of translational studies with adult stem cells. A major impediment to progress in the field of regenerative medicine is a lack of standardized methods to culture specific stem cells. Such documented methods would allow for scientific rigor and reproducibility to enhance efficient and safe translation of stem cells to patients. This book fills that gap.

Contents

Introduction: Core Techniques in Stem Cell Culture

1. Isolation of Hematopoietic Stem Cells

2. Isolation Protocol for CD133+ and CD34+ Very Small Embryonic Like Cells (VSEL) from Human Umbilical Cord Blood

3. Isolation and Expansion of Mesenchymal Stem Cells from the Adult Bone Marrow Aspirate

4. Manual Isolation and Expansion of Adipose Derived Stem Cells from Adipose Tissue

5. Isolating Compact Bone-derived Mesenchymal Stem Cells from Rodent and Rabbit Femurs

6. Isolating Dental Pulp Stem Cells

7. A Murine System for Somatic Cellular Reprogramming into Induced Pluripotent Stem Cells

8. Isolation and Expansion Of Placenta Stem Cells (P-MSCs)

9. Expanding Human Mesenchymal Stem Cells from the Umbilical Cord

10. Laboratory Isolation of Leukemia Cancer Stem Cell

11. Isolation and Characterization of Cancer Stem cells from Glioblastoma Multiforme and Breast Cancer Cell Lines

12. Bone Marrow Aspirate for Isolation of Mesenchymal Stem Cells

13. Culturing Human Bone Marrow Stromal Cells

14. Isolating Mononuclear Cells by Ficoll-Hypaque Density Gradient

15. Phenotypic and Multipotent Characterization of Bone Marrow-derived Mesenchymal Stem Cells

16. Cryopreservation of Relatively Small Number of Stem Cells

17. Cryopreservation of Mobilized Peripheral Blood Stem Cells

18. Manual Cell Count