Description
(Text)
Doctoral Thesis / Dissertation from the year 2004 in the subject Business economics - Supply, Production, Logistics, Szent István University (School of Veterinary Medicine), language: English, abstract: Inhaltsangabe:Abstract:
1. High prevalence of antibodies to soy antigens was demonstrated not only in swine and poultry sera, but also in commercial products as horse-radish labelled antibodies from goats, commercial complement preparations manufactured from guinea pig sera.
2. Upon heat treatment, which is recommended for the inactivation of the anti-nutritive components of soybeans, the formation of protein fibrils may occur. This fibril formation is of amyloid character, which may render the proteins involved resistant to proteolytic digestion.
3. One or more of the components of the conglycinin preparation have chaperone activity, ie. prevents the aggregation of proteins, thus reducing digestibility.
4. A ccording to laser particle sizer test protein A containing Staphylococcus aureus cells show substantial size reduction if the protein A bound antibodies are exposed to the appropriate antigens. This size reduction may also be responsible for dermatological diseases if circulating antigens reach antibody covered bacteria in the skin and the resulting size reduction leads to local, heamatological dissemination of the disrupted bacterial colonies (atopic dermatitis).
5. Amylase and xylanase reduce the amount of antigens in soybean, thus demonstrating the antigenic role of polysaccharides and one of the possible pathways of enzyme supplementation benefits. The method used for this demonstration can also used for optimizing enzyme combinations for feeds of different composition.
6. Soy overfeeding may lead to kidney and pancreas conditions which are characteristic of the protein overdosing.
7. Circulating antibodies to soy or soy components themselves do bind complement thus compromising the non-specific immunity of the host.
8. Soy antigens and Clostridial antigens may have interactions and/or sequence homologies with immunopathological implications.
Inhaltsverzeichnis:Inhaltsverzeichnis:
Summary2
Aim of the study5
The use of soybean8
Soy proteins10
Food products containing soy components10
Pricing of soybeans11
Receipts in the food industry13
Major components of soybean meals14
Trypsin inhibitors14
Lipase inhibitor15
Soybean lectin15
Lipoxygenase16
Glycinin16
Beta-Conglycinin17
Soybean Hydrophobic Protein18
Soybean Hull Protein (Gly m 2)18
Soybean Profilin (Gly m 3)18
Soybean Vacuolar Protein (Gly m Bd 30k)18
Soybean allergen nomenclature18
Soybean allergy19
Levels of soybean allergens20
Soy lipids and phytosterols20
Soy saponins20
Phytate21
Soyatoxin21
Soy phytoestrogens21
Carbohydrates and mucilage22
Polysaccharides and intestinal viscosity23
Materials and methods24
Results28
Separation of ASE components29
Effect of enzymes on soy extracts and preparations32
Amyloid formation and chaperone activity37
Anti-soy antibodies46
Bacterial tests52
Histology62
Pancreas62
Kidneys64
Thyroid65
Small intestine66
Wingweb68
Discussion69
Rodents69
Piglets70
Calves70
Chicks71
Antibodies, immunity and immune tolerance72
Relationship of Clostridial and soy antigens75
Staphylococcus and soybean proteins77
Amyloid formation and chaperone activity77
Enzymes and feeds78
Context: quality and biological activity79
Quality control80
References82
