Acta Haematologica. Vol.133/1 Human Hematopoietic Colonies in Health and Disease （2004. 92 p. w. 240 col. figs.）

Mitarbeit: Ramot, B./ Ed. by C. Nissen-Druey, A. Tichelli and S. Meyer-Monard

KARGER（2004発売）

• 製本 Hardcover:ハードカバー版／ページ数 96 p.／サイズ 230 fig., 229 in color.
• 商品コード 9783805578844

基本説明

Reprint of: 'Acta Haematologica 2005, Vol. 113, No. 1'.

Full Description

This atlas provides instructions for the culture of human hematopoietic precursor cells in methylcellulose. The morphology of live colonies from healthy individuals and patients with hematological disorders collected over the last 25 years is illustrated by 230 photographs. These photos provide evidence of the wide range of normality on the one hand, and demonstrate the recurrence of typical disease-associated growth patterns in different patients with the same disease on the other. The text focuses on the use of precursor cell cultures as a complementary tool for the diagnosis and follow-up of hematological stem cell disorders, highlighting their value in the differential diagnosis of hypoplastic and myeloproliferative disorders, with special emphasis on polycythemia vera, myelodysplastic syndromes, and chronic myelomonocytic leukemia. Practical and concise, this guide addresses clinical and experimental hematologists, technicians and teachers interested in human hematopoietic stem cells. Offering a unique collection of photographs taken at steady reproducible culture conditions by a hematologist with extensive clinical and experimental experience, it fills a gap in the current hematology literature.

Contents

Introduction Aims of the atlas Background The contribution of cultures in modern hematology Limitations of precursor cell cultures Culture techniques Laboratory equipment and materials Culture media Cell suspensions Optimal number of cells in culture Plating and incubation Reading of cultures Normality and abnormality in culture Normal colonies W CFU-G: Neutrophil granulocyte colonies W CFU-M and CFU-GM: Macrophage and granulocyte-macrophage col onies W CFU-Eo: Eosinophil colonies W BFU-E and CFU-E: Large and small erythroid colonies W CFU-GEMM: Mixed colonies containing neutrophil granulocytes, erythroblasts, macrophages and megakaryocytes W CFU Eo/Ec: Colonies containing eosinophils and erythroblast s W Normal colony numbers, PB:BM ratio and E:M ratio Deviations from normality W Grossly reduced colony growth W Strongly increased colony growth - with normal PB:BM ratio - with high PB:BM ratio W Low erythroid:myeloid (E:M) ratio W Increased CFU-M W Increased CFU-Eo W Increased CFU-GEMM and CFU-Eo/Ec W Deficient hemoglobinization W Erythropoetin-independent BFU-E W Abnormally small colonies W Dendritic cell (DC) - like clusters W Agglutinated red cells and platelets W Scattered macrophages W Non-cellular artefacts W Infectious contaminations W Lack of incubator humidity Contribution of cultures to clinical hematology Differential diagnosis of cytopenic disorders W Guideline from culture observation to diagnosis W Aplastic anemia W Myelodysplastic syndrome W Acute myeloid leukemia W Vitamin B12/folate deficiency W Agranulocytosis W Immune thrombocytopenia W Pure red cell aplasia W Severe Graft-versus-Host Disease Differential diagnosis of hyperproliferative disorders W Neutrophilia, polycythemia, thrombocytosis - Guide from culture observation to diagnosis - Polycythemia vera and essential - thrombocythemia - Idiopathic myelofibrosis - Chronic myeloid leukemia - Gleevec[registered] effects W Eosinophilia - Guide from culture observation to diagnosis - Reactive eosinophilia and hypereosinophilic - syndrome W Monocytosis - Guide from culture observation to diagnosis - Reactive monocytosis and chronic myelo- - monocytic leukemia Subject Index